Sun Wei, Chang Hong, Yang Zhangping, Geng Rongqing, Tsunoda Kenji, Ren Zhanjun, Chen Hongyu, Hussein Musa H
Animal Science & Technology College, Yangzhou University, Yangzhou 25009, China.
J Genet Genomics. 2007 Dec;34(12):1097-105. doi: 10.1016/S1673-8527(07)60125-8.
This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin (Tf), alkaline phosphatase (Alp), leucine aminopeptidase (Lap), arylesterase (Ary-Es), hemoglobin-beta (Hb-beta), X-protein (X-p), carbonic anhydrase (CA), catalase (Cat), malate dehydrogenase (MDH), and lysine (Ly), whereas, albumin (Al) and postalbumin (Po) loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the "Mongolia group", and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia, and was more effective in reflecting the breeding course of Tong sheep.
本研究以中国陕西省白水县中部典型群体随机抽样法获取的同羊为研究对象。开展调查以阐明同羊血液蛋白质和非蛋白质类型的基因组成。通过淀粉凝胶电泳和醋酸纤维素电泳检测了12个遗传标记。在同羊中发现了以下10个位点的多态性，即转铁蛋白（Tf）、碱性磷酸酶（Alp）、亮氨酸氨肽酶（Lap）、芳基酯酶（Ary-Es）、血红蛋白β（Hb-β）、X蛋白（X-p）、碳酸酐酶（CA）、过氧化氢酶（Cat）、苹果酸脱氢酶（MDH）和赖氨酸（Ly），而白蛋白（Al）和后白蛋白（Po）位点为单态。采用遗传距离法和系统发育关系聚类法判断同羊的起源和系统发育地位。两种方法的结果均表明同羊属于“蒙古羊组”，蒙古羊是同羊的起源。这也得到了同羊育种历史的支持。与系统发育关系聚类法相比，遗传距离法从东亚和中亚提取信息时更可靠，在反映同羊的育种历程方面更有效。
