Yu Hong, Li Qi
Fisheries College, Ocean University of China, Qingdao 266003, China.
J Genet Genomics. 2007 Dec;34(12):1114-22. doi: 10.1016/S1673-8527(07)60127-1.
Microsatellite DNA technique was used to detect the genetic variation between five hatchery populations of the Pacific oyster from China and two wild populations from Japan. Seven microsatellite loci screened in this study showed high polymorphism in both hatchery and wild populations, as observed in an average number of allele per locus (19.1-29.9) and average expected heterozygosity (0.916-0.958). No significant difference in average allelic richness or expected heterozygosity was observed between Chinese hatchery populations and Japanese wild populations. Pairwise FST values and heterogeneity tests of allele frequencies showed significant genetic differentiation between all populations. According to the neighbor-joining tree constructed on the basis of the DC distance, the seven populations fell into three groups showing a clear division between hatchery and wild populations, and between the northern and southern hatchery populations. Assignment tests correctly assigned high percentages (97%-100%) of individuals to their original populations and demonstrated the feasibility of microsatellite analysis for discrimination between populations. The information obtained in this study is useful for designing suitable management guidelines and selective breeding programs for the Pacific oyster in China.
采用微卫星DNA技术检测了中国五个太平洋牡蛎养殖群体与日本两个野生群体之间的遗传变异。本研究筛选的7个微卫星位点在养殖群体和野生群体中均表现出高度多态性，每个位点的平均等位基因数为19.1 - 29.9，平均期望杂合度为0.916 - 0.958。中国养殖群体和日本野生群体之间的平均等位基因丰富度或期望杂合度没有显著差异。成对FST值和等位基因频率的异质性检验表明所有群体之间存在显著的遗传分化。根据基于DC距离构建的邻接树，这七个群体分为三组，显示出养殖群体与野生群体之间以及北方与南方养殖群体之间的明显分化。分配测试将高比例（97% - 100%）的个体正确分配到其原始群体，并证明了微卫星分析用于群体间鉴别的可行性。本研究获得的信息有助于为中国太平洋牡蛎设计合适的管理指南和选育计划。
