Ouji Yukiteru, Yoshikawa Masahide, Moriya Kei, Nishiofuku Mariko, Matsuda Ryosuke, Ishizaka Shigeaki
Program in Tissue Engineering and Department of Parasitology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
Biochem Biophys Res Commun. 2008 Mar 7;367(2):299-304. doi: 10.1016/j.bbrc.2007.12.091. Epub 2007 Dec 26.
Although Wnts are expressed in hair follicles throughout life from embryo to adult, and considered to be critical for their development and maturation, their roles remain largely unknown. In the present study, we investigated the effects of Wnts (Wnt-3a, Wnt-5a, Wnt-10b, and Wnt-11) on epithelial cell differentiation using adult mouse-derived primary skin epithelial cell (MPSEC) cultures and hair growth using hair follicle organ cultures. Only Wnt-10b showed evident promotion of epithelial cell differentiation and hair shaft growth, in contrast to Wnt-3a, 5a, and 11. Our results suggest that Wnt-10b is unique and plays an important role in differentiation of epithelial cells in the hair follicle.
尽管Wnts在从胚胎到成年的整个生命周期中都在毛囊中表达，并被认为对其发育和成熟至关重要，但其作用在很大程度上仍不清楚。在本研究中，我们使用成年小鼠来源的原代皮肤上皮细胞（MPSEC）培养物研究了Wnts（Wnt-3a、Wnt-5a、Wnt-10b和Wnt-11）对上皮细胞分化的影响，并使用毛囊器官培养物研究了其对毛发生长的影响。与Wnt-3a、5a和11相比，只有Wnt-10b显示出对上皮细胞分化和毛干生长有明显的促进作用。我们的结果表明，Wnt-10b是独特的，并且在毛囊上皮细胞的分化中起重要作用。
