Department of Cell Biology, Third Military Medical University, ShaPingBa, Chongqing, China.
Clin Exp Dermatol. 2011 Jul;36(5):534-40. doi: 10.1111/j.1365-2230.2011.04019.x. Epub 2011 Mar 11.
Wnt10b (wingless-related mouse mammary tumour virus integration site 10b) plays various roles in a wide range of biological actions, including hair-follicle development.
To assess the roles that Wnt10b plays in postnatal hair-follicle growth.
Adenovirus vectors AdWnt10b, AdGFP, AdGFP plus AdRFP, AdWnt10b plus AdFrzB, and AdWnt10b plus AdSimBC were co-cultured separately with vibrissae. In situ protein expression of Wnt10b, β-catenin and Lef1 was determined by immunohistochemistry, and the proliferation status of the hair follicle was detected by 5-bromo-2-deoxyuridine (BrdU) labelling. The presence of Wnt signalling molecules in the three stages of hair-follicle growth was detected by PCR-based microarray.
AdWnt10b-infected cells were able to secrete bioactive Wnt10b, and when this was added into the basal medium, the vibrissae grew faster than in control medium or in medium containing canonical Wnt signalling antagonists. The in situ protein expression of Wnt10b was consistent with that of β-catenin and Lef1. The expression locus of Wnt10b was almost the same as the proliferating cells labelled by BrdU in the anagen hair follicle.
Wnt10b may promote hair-follicle growth by inducing the switch from telogen to anagen via a canonical Wnt signalling pathway to promote the proliferation of matrix cells.
Wnt10b（无翅型小鼠乳腺肿瘤病毒整合位点 10b）在广泛的生物学作用中发挥着各种作用，包括毛发生长。
评估 Wnt10b 在出生后毛囊生长中的作用。
腺病毒载体 AdWnt10b、AdGFP、AdGFP 加 AdRFP、AdWnt10b 加 AdFrzB 和 AdWnt10b 加 AdSimBC 分别与触须共培养。免疫组织化学法检测 Wnt10b、β-catenin 和 Lef1 的原位蛋白表达，5-溴-2-脱氧尿苷（BrdU）标记检测毛囊的增殖状态。基于 PCR 的微阵列检测毛囊生长三个阶段中 Wnt 信号分子的存在。
感染 AdWnt10b 的细胞能够分泌生物活性 Wnt10b，当将其添加到基础培养基中时，触须的生长速度比对照培养基或含有经典 Wnt 信号通路拮抗剂的培养基中更快。Wnt10b 的原位蛋白表达与 β-catenin 和 Lef1 的表达一致。Wnt10b 的表达部位与 BrdU 标记的生长期毛囊中的增殖细胞几乎相同。
Wnt10b 可能通过经典 Wnt 信号通路诱导从休止期到生长期的转变，从而促进基质细胞的增殖，从而促进毛囊生长。
