马铃薯X病毒粒子中另一种可能的结构转变及病毒衣壳蛋白结构的修订模型。
One more probable structural transition in potato virus X virions and a revised model of the virus coat protein structure.
作者信息
Nemykh Maria A, Efimov Alexander V, Novikov Victor K, Orlov Victor N, Arutyunyan Alexander M, Drachev Vladimir A, Lukashina Elena V, Baratova Lyudmila A, Dobrov Evgeny N
机构信息
Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119991, Russia.
出版信息
Virology. 2008 Mar 30;373(1):61-71. doi: 10.1016/j.virol.2007.11.024. Epub 2007 Dec 26.
We found that a 2-h incubation of potato virus X (PVX) virions in 10 mM Tris-HCl buffer pH 7.5 at -20 degrees C results in a strong but reversible drop in virion stability. Under these conditions, the PVX virions are completely disrupted by low (starting from 50 mM) concentrations of LiCl and CaCl(2) but not of NaCl. Incubation of PVX samples with 0.05-2 M LiCl at +4 degrees C did not result in virion disassembly and the virions were not disrupted upon incubation at -20 degrees C in 10 mM Tris-HCl buffer pH 7.5 without LiCl. We suggest that a 2-h incubation of the PVX virions at -20 degrees C in 10 mM Tris-HCl pH 7.5 results in a structural transition in the virus particles. A revised model of the three-dimensional organization of coat protein subunits in the PVX virions is proposed. This two-domain model explains better the high plasticity of the PVX CP structure.
我们发现，马铃薯X病毒（PVX）病毒粒子在pH 7.5的10 mM Tris-HCl缓冲液中于-20℃孵育2小时会导致病毒粒子稳定性显著但可逆地下降。在这些条件下，PVX病毒粒子会被低浓度（从50 mM开始）的LiCl和CaCl₂完全破坏，但不会被NaCl破坏。PVX样品在+4℃下与0.05 - 2 M LiCl孵育不会导致病毒粒子解体，并且在不含LiCl的10 mM Tris-HCl缓冲液pH 7.5中于-20℃孵育时病毒粒子也不会被破坏。我们认为，PVX病毒粒子在10 mM Tris-HCl pH 7.5中于-20℃孵育2小时会导致病毒颗粒发生结构转变。提出了PVX病毒粒子中衣壳蛋白亚基三维组织的修订模型。这个双结构域模型更好地解释了PVX CP结构的高可塑性。
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