Zhao Yang, Sun Yan, Li Chuan
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
J Am Soc Mass Spectrom. 2008 Mar;19(3):445-9. doi: 10.1016/j.jasms.2007.11.015. Epub 2007 Nov 29.
Extracts from Ginkgo biloba leaves confer their therapeutic effects through the synergistic actions of flavonoid and terpenoid components. We herein describe the development of an LC-MS/MS-based method for simultaneous determination of flavonoids (quercetin, kaempferol, and isorhamnetin) and terpenoids (bilobalide, ginkgolides A, B, C, and J) in acid-hydrolyzed plasma by circumventing cross-interference between the flavonoids and terpenoids identified. Notably, inclusion of ammonium formate (0.2 mM) in the mobile phase generated beneficial LC-electrolyte effects, including increased ESI efficiency and capacity, with the result that the newly developed procedure exhibits the highest analytical performance reported to date for ginkgo-associated studies. The method yields high sensitivity, negligible matrix interference and cross-interference, wide linear dynamic ranges, high sample throughput, and quite small initial sample size. The assay utility to dog pharmacokinetic measurements of commercial ginkgo products yielded the most comprehensive data on systemic exposure to the ginkgo compounds to date. The newly developed multi-analyte procedure should be widely useful.
银杏叶提取物通过黄酮类和萜类成分的协同作用发挥其治疗效果。我们在此描述了一种基于液相色谱-串联质谱法的方法的开发，该方法用于同时测定酸水解血浆中的黄酮类化合物（槲皮素、山奈酚和异鼠李素）和萜类化合物（白果内酯、银杏内酯A、B、C和J），通过规避所鉴定的黄酮类化合物和萜类化合物之间的交叉干扰。值得注意的是，在流动相中加入甲酸铵（0.2 mM）产生了有益的液相色谱-电解质效应，包括提高电喷雾电离（ESI）效率和容量，结果是新开发的方法在迄今报道的银杏相关研究中表现出最高的分析性能。该方法具有高灵敏度、可忽略不计的基质干扰和交叉干扰、宽线性动态范围、高样品通量以及非常小的初始样品量。该分析方法在犬类对商业银杏产品的药代动力学测量中的应用产生了迄今为止关于银杏化合物全身暴露的最全面数据。新开发的多分析物方法应该具有广泛的用途。
