Serra Moreno J, Panero S, Artico M, Filippini P
Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Bioelectrochemistry. 2008 Feb;72(1):3-9. doi: 10.1016/j.bioelechem.2007.11.002. Epub 2007 Nov 13.
Novel composite polypyrrole/chondroitin-4-sulphate films with cation-exchange properties were synthesized by the electrochemical polymerization of pyrrole in the presence of chondroitin-4-sulphate (CSA) sodium salt, acting as dopant anion at neutral pH. The negatively charged biomolecule was found to be permanently entrapped in the polypyrrole (PPy) membrane which resulted, as expected, facilitated in the mass transport by mobile cationic counterions. The porous nature of the substrates was identified as the most influential factor controlling the morphology. The morphology, in turn, affects the interaction between the material surface and the tissues on a cellular level. In this work in vitro analyses of human fibroblast response to polypyrrole/chondroitin-4-sulphate films were performed to focus on the different steps of cell reactions towards defined surface properties.
通过在中性pH值下，以硫酸软骨素-4钠盐（CSA）作为掺杂阴离子，在其存在的情况下进行吡咯的电化学聚合反应，合成了具有阳离子交换特性的新型复合聚吡咯/硫酸软骨素-4薄膜。发现带负电荷的生物分子被永久地截留在聚吡咯（PPy）膜中，正如预期的那样，移动的阳离子抗衡离子促进了物质传输。基底的多孔性质被确定为控制形态的最有影响的因素。反过来，形态又在细胞水平上影响材料表面与组织之间的相互作用。在这项工作中，对人成纤维细胞对聚吡咯/硫酸软骨素-4薄膜的反应进行了体外分析，以关注细胞对特定表面性质反应的不同步骤。
