Margiolaki I, Wright J P
European Synchrotron Radiation Facility (ESRF), Grenoble, France.
Acta Crystallogr A. 2008 Jan;64(Pt 1):169-80. doi: 10.1107/S0108767307043735. Epub 2007 Dec 21.
Following the seminal work of Von Dreele, powder X-ray diffraction studies on proteins are being established as a valuable complementary technique to single-crystal measurements. A wide range of small proteins have been found to give synchrotron powder diffraction profiles where the peak widths are essentially limited only by the instrumental resolution. The rich information contained in these profiles, combined with developments in data analysis, has stimulated research and development to apply the powder technique to microcrystalline protein samples. In the present work, progress in using powder diffraction for macromolecular crystallography is reported.
继冯·德雷尔的开创性工作之后，蛋白质的粉末X射线衍射研究正成为一种有价值的补充技术，可用于单晶测量。人们发现，许多小蛋白质能给出同步辐射粉末衍射图谱，其峰宽基本上仅受仪器分辨率限制。这些图谱中包含的丰富信息，再加上数据分析的进展，激发了将粉末技术应用于微晶蛋白质样品的研究与开发。在本工作中，报告了在将粉末衍射用于大分子晶体学方面取得的进展。
