International Goods Inspection Company, Tehran, Iran.
J Sep Sci. 2011 Jun;34(11):1333-7. doi: 10.1002/jssc.201000882. Epub 2011 Apr 13.
A simple and rapid method based on homogeneous liquid-liquid extraction coupled to HPLC with fluorescence detection was developed for the determination of aflatoxin B1 (AFB1) in the rice and grain samples after post-column derivatization. The proposed method eliminated the use of immunoaffinity columns for clean-up in the determination of AFB1. The parameters affecting recovery and preconcentration such as type and volume of organic solvent, volume ratio of water/methanol, concentration of phase separator reagent and extraction time were optimized. Under the optimized conditions, the calibration graph was linear in the concentration range of 0.01-1.0 ng/g with the detection limit of 0.003 ng/g. This method was successfully applied for the analysis of AFB1 in different cereal samples.
建立了一种基于均相液-液萃取结合高效液相色谱-荧光检测的简单快速方法，用于柱后衍生化后大米和谷物样品中黄曲霉毒素 B1（AFB1）的测定。该方法在测定 AFB1 时无需使用免疫亲和柱进行净化。优化了影响回收率和浓缩的参数，如有机溶剂的类型和体积、水/甲醇体积比、相分离试剂的浓度和萃取时间。在优化条件下，校准曲线在 0.01-1.0ng/g 浓度范围内呈线性，检测限为 0.003ng/g。该方法成功应用于不同谷物样品中 AFB1 的分析。
