Laboratorio de Parasitología, Centro de Investigaciones Agrarias de Mabegondo, Instituto Galego de Calidade Alimentaria-Xunta de Galicia, Carretera AC-542 de Betanzos a Mesón do Vento, Km 7,5, CP 15318, Abegondo (A Coruña), Spain.
Prev Vet Med. 2011 Aug 1;101(1-2):58-64. doi: 10.1016/j.prevetmed.2011.04.019. Epub 2011 Jun 6.
A comprehensive field study was carried out with the following objectives: (a) to assess the usefulness of individual and bulk tank milk analysis for determining Neospora caninum serostatus in individual cows and herds, and (b) to study the associations between N. caninum infection status (based on milk testing), and several productive and reproductive parameters in the animals. Antibodies were detected with a commercially available ELISA test (Bio K 192/5). Analysis of paired serum and milk samples from 1134 lactating cows on 38 farms revealed that 97.6% of the ELISA results were coincident, irrespective of whether serum or milk samples were used. Moreover, multiple linear regression analysis revealed that 86.0% of the variations in ELISA values in milk were due to variations in the serum. The measurement of antibodies in bulk tank milk was a good estimator of the herd level status of N. caninum infection, and enabled detection of infection in 94.7% herds with ≥10.0% seropositive cows and/or in all herds with >4% highly seropositive cows. The odds ratio for abortion in seropositive animals was 9.1 times higher than in seronegative animals. The infection serostatus was also a significant risk factor, as the odds ratio for abortion was even higher (12.0 times) in cows categorized as highly seropositive. ELISA values for the bulk milk from 387 randomly selected herds were negatively associated with average milk production. Moreover, milk production losses mainly occurred on farms categorized as highly positive (i.e. herds with ≥20.0% seropositive cows).
进行了一项全面的现场研究，目的如下：(a) 评估个体和牛群奶样分析在确定个体牛和牛群中有无新孢子虫血清阳性的用途，(b) 研究基于牛奶检测的新孢子虫感染状态与动物的几个生产和繁殖参数之间的关联。使用市售的 ELISA 检测法（Bio K 192/5）检测抗体。对 38 个牧场的 1134 头泌乳牛的配对血清和奶样进行分析，结果显示，无论使用血清还是奶样，97.6%的 ELISA 结果是一致的。此外，多元线性回归分析表明，奶中 ELISA 值的 86.0%的变化是由血清的变化引起的。牛群奶中抗体的检测是新孢子虫感染牛群水平状态的良好估计指标，可检测到 94.7%的牛群中有≥10.0%的血清阳性牛和/或所有牛群中有>4%的高度血清阳性牛发生感染。血清阳性动物的流产比值比为血清阴性动物的 9.1 倍。感染血清阳性状态也是一个重要的风险因素，因为分类为高度血清阳性的牛的流产比值比甚至更高（12.0 倍）。387 个随机选择的牛群的牛群奶的 ELISA 值与平均产奶量呈负相关。此外，产奶量的损失主要发生在被归类为高度阳性的农场（即牛群中有≥20.0%的血清阳性牛）。
