Rocha Rick, Silveira Landulfo, Villaverde Antonio Balbin, Pasqualucci Carlos A, Costa Maricilia S, Brugnera Aldo, Pacheco M T T
Institute of Research and Development, Universidade do Vale do Paraíba, São José dos Campos, Universidade de São Paulo, São Paulo, SP, Brazil.
Photomed Laser Surg. 2007 Dec;25(6):482-6. doi: 10.1089/pho.2007.2111.
The aim of this work was to identify the presence of atherosclerotic plaque in the human carotid artery using near infrared Raman spectroscopy.
Atherosclerosis is the most common and serious pathology of the cardiovascular system. Raman spectroscopy is an analytical tool that can be used to gather information about both the morphology and chemical composition of tissues.
A Ti:sapphire laser operating at the near-infrared wavelength of 830 nm pumped by an argon laser was used for excitation of the samples, and the Raman scattering was detected by an optical spectrometer with a liquid-nitrogen-cooled CCD detector. Carotid artery samples were classified into five groups: normal, intimal thickening, fatty plaque, fibrous-fatty plaque, and fibrous-calcified plaque.
It was observed that the Raman spectrum of atheromatous plaque was different that that of normal tissue. The spectra of atheromatous plaques had bands due to the presence of cholesterol and its esters, with major bands at 1439 and 1663 cm(1), respectively. In normal tissues a peak related to C-H bending appears at 1451 cm(1). Calcified atheromatous plaques had primary bands at 961 and 1071 cm(1), which were due to the presence of phosphate and carbonate in the accumulated calcium. Peaks were seen at 1451 and 1655 cm(1) in the non-atherosclerotic tissue, which were shifted to 1439 and 1663 cm(1) in the atherosclerotic plaque.
Our results indicate that this technique could be used to detect the presence of atherosclerotic plaques in carotid arterial tissue.
本研究旨在利用近红外拉曼光谱法识别人类颈动脉中动脉粥样硬化斑块的存在。
动脉粥样硬化是心血管系统最常见且最严重的病理状况。拉曼光谱法是一种分析工具，可用于收集有关组织形态和化学成分的信息。
使用由氩离子激光泵浦的波长为830 nm的近红外钛宝石激光器对样品进行激发，并通过配备液氮冷却电荷耦合器件（CCD）探测器的光学光谱仪检测拉曼散射。颈动脉样本分为五组：正常、内膜增厚、脂肪斑块、纤维脂肪斑块和纤维钙化斑块。
观察到动脉粥样硬化斑块的拉曼光谱与正常组织不同。动脉粥样硬化斑块的光谱因胆固醇及其酯类的存在而有谱带，主要谱带分别位于1439和1663 cm⁻¹处。在正常组织中，与C-H弯曲相关的峰出现在1451 cm⁻¹处。钙化的动脉粥样硬化斑块在961和1071 cm⁻¹处有主要谱带，这是由于积累的钙中存在磷酸盐和碳酸盐。在非动脉粥样硬化组织中，在1451和1655 cm⁻¹处出现峰，在动脉粥样硬化斑块中这些峰移至1439和1663 cm⁻¹处。
我们的结果表明，该技术可用于检测颈动脉组织中动脉粥样硬化斑块的存在。
