Faculty of Engineering, Department of Chemistry, Istanbul University-Cerrahpaşa, Avcilar, 34320, Istanbul, Turkey.
Faculty of Science, Department of Biology, Istanbul University, Vezneciler, 34134, Istanbul, Turkey.
Biol Trace Elem Res. 2022 Feb;200(2):720-727. doi: 10.1007/s12011-021-02682-6. Epub 2021 Mar 26.
The current study was designed to investigate the effects of zinc sulfate on cell proliferation, metallothionein (MT) immunoreactivity and antioxidant system against acute ethanol-induced oxidative damage in tongue tissues of rats. Wistar albino male rats, 2.5 to 3.0 months, were divided into four groups: Group I (n = 8), intact control rats; group II (n = 8), control animals given only zinc sulfate (100 mg/kg/day, for 3 consecutive days); group III (n = 14), animals given 1 mL absolute ethanol; group IV (n = 11), animals given zinc sulfate and absolute ethanol at the same dose and time. Animals were sacrificed under anesthesia 2 h after ethanol administration or 4 h after the last zinc sulfate treatment. Ethanol administration caused a marked decrease in the number of MT immunopositive cells and the proliferating cells in the lingual epithelium. A statistically significant decline in reduced glutathione levels, catalase activity and superoxide dismutase activities was also observed, whereas a significant elevation of lipid peroxidation levels and lactate dehydrogenase activities was detected in the ethanol group. In contrast, these changes were reversed by administration of zinc sulfate to ethanol-treated rats. In conclusion, it shows that zinc sulfate has therapeutic effects on acute ethanol-induced oxidative damage in the tongue tissues of rats.
本研究旨在探讨硫酸锌对急性乙醇诱导的大鼠舌组织氧化损伤的细胞增殖、金属硫蛋白（MT）免疫反应和抗氧化系统的影响。将 2.5 至 3.0 个月大的 Wistar 白化雄性大鼠分为四组：第 I 组（n = 8），完整对照组大鼠；第 II 组（n = 8），仅给予硫酸锌（100mg/kg/天，连续 3 天）的对照组动物；第 III 组（n = 14），给予 1ml 无水乙醇的动物；第 IV 组（n = 11），给予相同剂量和时间的硫酸锌和无水乙醇的动物。乙醇给药后 2 小时或最后一次硫酸锌处理后 4 小时，动物在麻醉下处死。乙醇给药导致舌上皮中 MT 免疫阳性细胞和增殖细胞的数量明显减少。还观察到还原型谷胱甘肽水平、过氧化氢酶活性和超氧化物歧化酶活性显著下降，而乙醇组的脂质过氧化水平和乳酸脱氢酶活性显著升高。相反，这些变化在给予乙醇处理的大鼠硫酸锌后得到逆转。总之，结果表明硫酸锌对急性乙醇诱导的大鼠舌组织氧化损伤具有治疗作用。
