Warda Mohamad, Zhang Fuming, Radwan Moustafa, Zhang Zhenqing, Kim Nari, Kim Young Nam, Linhardt Robert J, Han Jin
Biochemistry Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Glycoconj J. 2008 Jul;25(5):441-50. doi: 10.1007/s10719-007-9090-8. Epub 2007 Dec 27.
Impaired placento-fetal communication is a coherent symptom of exaggerated pre-eclampsia. The impact of the cellular expression of different glycosaminoglycans (GAGs) in this event on the placenta in pre-eclampsia is still obscure. This is the first study aimed at discovering the relationship between structural alterations of different sulfated GAGs at the molecular level and the development of pre-eclampsia in inflicted placenta. Sulfated GAGs were isolated and purified from control and pre-eclampsia placentas. The amount and the molecular weight of GAG in each tissue sample were measured. The polydispersity of the recovered GAG samples were determined by polyacrylamide gel electrophoresis. The disaccharide composition of chondroitin sulfate, dermatan sulfate and heparan sulfate were deduced by chondroitinase and heparinase depolymerization followed by liquid chromatography-mass spectrometry. The in vivo sulfo-modulation of GAGs in pre-eclampsia and control placenta were examined using RT-PCR to determine the transcription levels of different sulfotransferases involved in GAG biosynthesis. Marked differences in GAG sulfation patterns and mRNA level of encoding selected GAG O-sulfotransferases were observed in pre-eclampsia. These data suggest a linkage between pre-eclampsia and the observed alterations in placental GAGs and could provide new insights about the modulating role of GAGs in the development and the severity of placental pre-eclampsia.
胎盘 - 胎儿间通讯受损是重度子痫前期的一个连贯症状。不同糖胺聚糖（GAGs）的细胞表达在此过程中对子痫前期胎盘的影响仍不清楚。这是第一项旨在在分子水平上发现不同硫酸化GAGs的结构改变与受累胎盘子痫前期发展之间关系的研究。从对照胎盘和子痫前期胎盘中分离并纯化硫酸化GAGs。测量每个组织样品中GAG的量和分子量。通过聚丙烯酰胺凝胶电泳测定回收的GAG样品的多分散性。通过软骨素酶和肝素酶解聚，然后进行液相色谱 - 质谱法推导硫酸软骨素、硫酸皮肤素和硫酸乙酰肝素的二糖组成。使用逆转录 - 聚合酶链反应（RT-PCR）检测子痫前期和对照胎盘中GAGs的体内磺基调节，以确定参与GAG生物合成的不同磺基转移酶的转录水平。在子痫前期中观察到GAG硫酸化模式和编码选定GAG O - 磺基转移酶的mRNA水平存在明显差异。这些数据表明子痫前期与观察到的胎盘GAGs改变之间存在联系，并可为GAGs在胎盘子痫前期发展和严重程度中的调节作用提供新的见解。
