Bedran-Russo Ana Karina B, Pashley David H, Agee Kelli, Drummond James L, Miescke Klaus J
Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois, USA.
J Biomed Mater Res B Appl Biomater. 2008 Aug;86(2):330-4. doi: 10.1002/jbm.b.31022.
It is thought that increasing the strength of the dentin matrix using crosslinking agents may improve both the strength and the durability of resin-dentin bonds. The purpose of this study was to evaluate the effect of two collagen crosslinking agents (glutaraldehyde, GD and grape seed extract, GSE) on the modulus of elasticity of demineralized dentin. Sound molar fragments were fully demineralized and divided into five groups according to the type and concentration of crosslinking agents: 2.5% GD; 5% GD, 25% GD; 0.65% GSE; 6.5% GSE. Specimens were immersed in their respective solution and tested at baseline, 10 min, 30 min, 1 h, 2 h, 4 h. The elastic modulus of dentin was significantly affected by the treatment (p < 0.01) and exposure time (p < 0.01). There was a statistically significant interaction between the two factors evaluated (treatment vs. time p < 0.01). Mean baselines values varied between 4.8 and 6.2 MPa in water; after 4 h of treatment the values increased between 34.9 and 242.5 MPa, that were treatment time and agent dependent. The use of these collagen crosslinkers to increase the stiffness of demineralized dentin, was both concentration and time dependent.
人们认为，使用交联剂增强牙本质基质的强度可能会提高树脂与牙本质结合的强度和耐久性。本研究的目的是评估两种胶原蛋白交联剂（戊二醛，GD和葡萄籽提取物，GSE）对脱矿牙本质弹性模量的影响。将完好的磨牙碎片完全脱矿，并根据交联剂的类型和浓度分为五组：2.5% GD；5% GD，25% GD；0.65% GSE；6.5% GSE。将标本浸入各自的溶液中，并在基线、10分钟、30分钟、1小时、2小时、4小时进行测试。牙本质的弹性模量受处理（p < 0.01）和暴露时间（p < 0.01）的显著影响。所评估的两个因素（处理与时间p < 0.01）之间存在统计学上的显著相互作用。在水中，平均基线值在4.8至6.2 MPa之间变化；处理4小时后，值在34.9至242.5 MPa之间增加，这取决于处理时间和试剂。使用这些胶原蛋白交联剂来增加脱矿牙本质的硬度，是浓度和时间依赖性的。
