Eymirli Pınar Serdar, Gültekin İrem Mergen, Özler Cansu Özşin, Özyürek Emel Uzunoğlu
Department of Pediatric Dentistry, Faculty of Dentistry, Hacettepe University, Altındağ/Ankara, Turkey.
Department of Pediatric Dentistry, Faculty of Dentistry, Lokman Hekim University, Altındağ/Ankara, Turkey.
J Dent Res Dent Clin Dent Prospects. 2024 Summer;18(3):182-188. doi: 10.34172/joddd.41348. Epub 2024 Sep 7.
This study evaluated the efficacy of grape seed extract (GSE) on the remineralization of primary tooth enamel alone or in combination with remineralizing agents.
The initial microhardness value of 90 primary tooth enamel samples was calculated; then, the samples were demineralized. The post-demineralization hardness of the samples was measured and the samples were randomly divided into 6 groups as follows: G1: negative control, G2: GSE, G3: NaF, G4:Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), G5: GSE+NaF, and G6: GSE+CPP-ACP (n=15). Oral environment pH cycle was applied and hardness measurements were repeated after treatments. The samples were stained with 1% rhodamine B dye and sectioned, and the lesion depth was measured. Statistical significance was set at <0.05.
The hardness decrease of the GSE and GSE+NaF groups was less than the other groups (<0.05). The decrease was also less in the other groups than in the control group (>0.05). GSE showed a positive effect when combined with NaF in maintaining microhardness but did not show the same effect when combined with CPP-ACP (<0.05). Concerning penetration depth, all the groups had statistically lower values than the control group (<0.05). The lowest penetration rates were observed in the GSE+NaF and GSE+CPP-ACP groups (<0.05).
The lowest hardness decrease was observed in the GSE and GSE+NaF groups, and the lowest penetration rates were observed in the GSE+NaF and GSE+CPP-ACP groups. It has been determined that a 15% GSE solution might be used as an alternative to fluoride in primary tooth remineralization and can increase the effectiveness of fluoride when used together.
本研究评估了葡萄籽提取物（GSE）单独或与再矿化剂联合使用对乳牙釉质再矿化的效果。
计算90个乳牙釉质样本的初始显微硬度值；然后，将样本进行脱矿处理。测量样本脱矿后的硬度，并将样本随机分为6组，如下：G1：阴性对照组，G2：GSE组，G3：氟化钠（NaF）组，G4：酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）组，G5：GSE+NaF组，G6：GSE+CPP-ACP组（n=15）。应用口腔环境pH循环，并在处理后重复进行硬度测量。用1%罗丹明B染料对样本进行染色并切片，测量病变深度。设定统计学显著性水平为<0.05。
GSE组和GSE+NaF组的硬度下降幅度小于其他组（<0.05）。其他组的下降幅度也小于对照组（>0.05）。GSE与NaF联合使用时在维持显微硬度方面显示出积极效果，但与CPP-ACP联合使用时未显示出相同效果（<0.05）。关于渗透深度，所有组在统计学上的值均低于对照组（<0.05）。在GSE+NaF组和GSE+CPP-ACP组中观察到最低的渗透率（<0.05）。
在GSE组和GSE+NaF组中观察到最低的硬度下降，在GSE+NaF组和GSE+CPP-ACP组中观察到最低的渗透率。已确定15%的GSE溶液可作为乳牙再矿化中氟化物的替代品，并且与氟化物一起使用时可提高其有效性。
