School of Dentistry, University of Missouri - Kansas City, Kansas City, MO 64108, USA.
School of Dentistry, University of Missouri - Kansas City, Kansas City, MO 64108, USA.
Dent Mater. 2023 Nov;39(11):1004-1012. doi: 10.1016/j.dental.2023.09.002. Epub 2023 Sep 19.
To develop dual-functional etchants that could demineralize and stabilize dentin collagen simultaneously, and to assess the effects of these etchants on collagen crosslinking, biostability and resin bonding properties under clinically relevant conditions.
Dual-functional etchants were prepared by mixing 56% glycolic acid and 17% phosphoric acid and adding 1% of theaflavins (TF) or proanthocyanidins from grape seed extract (GSE). The etchant without crosslinker was used as control. The prepared human dentin specimens were treated with the 3 etchants for 30 s and analyzed for chemical interaction using Fourier transform infrared spectroscopy and resistance of the demineralized layer to collagenase degradation using electron microscopy (EM). Resin-dentin interfacial bonding properties were evaluated after 24 h and after 10,000 thermocycling through microtensile bond strength (μTBS), nanoleakage and matrix metalloproteinases (MMPs) activity via in situ zymography. Statistical analysis was done using ANOVA and post- hoc Tuckey's test.
Compared to control, TF and GSE dual-functional etchants were able to demineralize dentin, induce collagen crosslinking and protect the demineralized layer from collagenase degradation within 30 s. High resolution EM images showed better protection with TF etchant compared to GSE. There was a significant reduction in μTBS and an increase in nanoleakage and MMPs activity in control after thermocycling (p < 0.05) while these changes weren't seen in dual-functional etchants.
Dual-functional etchants, especially TF containing, provide collagen protection against degradation and result in stable μTBS and less nanoleakage and MMPs activity under clinically relevant conditions.
开发既能脱矿又能同时稳定牙本质胶原的双功能蚀刻剂，并评估这些蚀刻剂在临床相关条件下对胶原交联、生物稳定性和树脂粘结性能的影响。
通过混合 56%的乙二醇酸和 17%的磷酸，并添加 1%的茶黄素（TF）或葡萄籽提取物中原花青素（GSE），制备双功能蚀刻剂。不含交联剂的蚀刻剂作为对照。用 3 种蚀刻剂处理 30s 后的人牙本质标本，用傅里叶变换红外光谱法分析化学相互作用，用电子显微镜（EM）分析脱矿层对胶原酶降解的抵抗力。在 24 小时和 10000 次热循环后，通过微拉伸粘结强度（μTBS）、纳米泄漏和基质金属蛋白酶（MMPs）活性的原位酶谱法评估树脂-牙本质界面粘结性能。使用方差分析和事后 Tukey 检验进行统计分析。
与对照组相比，TF 和 GSE 双功能蚀刻剂在 30s 内即可脱矿牙本质、诱导胶原交联并保护脱矿层免受胶原酶降解。高分辨率 EM 图像显示，与 GSE 相比，TF 蚀刻剂具有更好的保护作用。热循环后，对照组的 μTBS 显著降低，纳米泄漏和 MMPs 活性增加（p<0.05），而双功能蚀刻剂中没有出现这些变化。
双功能蚀刻剂，特别是含 TF 的，可提供胶原保护，防止降解，并在临床相关条件下产生稳定的 μTBS 和较少的纳米泄漏和 MMPs 活性。
