Shelton Shirley B, Pettigrew David B, Hermann Alison D, Zhou Weidong, Sullivan Patrick M, Crutcher Keith A, Strauss Kenneth I
Department of Neurosurgery, Division of Research, University of Cincinnati, 231 Albert Sabin Way, Medical Science Building, Cincinnati, OH 45267-0515, United States.
J Neurosci Methods. 2008 Mar 15;168(2):431-42. doi: 10.1016/j.jneumeth.2007.11.003. Epub 2007 Nov 19.
A refined battery of neurological tests, SNAP (Simple Neuroassessment of Asymmetric Impairment), was developed and validated to efficiently assess neurological deficits induced in a mouse model of traumatic brain injury. Four to 7-month old mice were subjected to unilateral controlled cortical impact or sham injury (craniectomy only). Several behavioral tests (SNAP, beam walk, foot fault, and water maze) were used to assess functional deficits. SNAP was unique among these in that it required no expensive equipment and was performed in less than 5 min per mouse. SNAP demonstrated a high level of sensitivity and specificity as determined by receiver-operator characteristics curve analysis. Interrater reliability was good, as determined by Cohen's Kappa method and by comparing the sensitivity and specificity across various raters. SNAP detected deficits in proprioception, visual fields, and motor strength in brain-injured mice at 3 days, and was sensitive enough to detect magnitude and recovery of injury. The contribution of individual battery components changed as a function of time after injury, however, each was important to the overall SNAP score. SNAP provided a sensitive, reliable, time-efficient and cost-effective means of assessing neurological deficits in mice after unilateral brain injury.
一种经过改进的神经学测试组合，即SNAP（不对称损伤简易神经评估），被开发并验证，用于有效评估创伤性脑损伤小鼠模型中诱发的神经功能缺损。对4至7月龄小鼠进行单侧控制性皮质撞击或假手术损伤（仅颅骨切除术）。使用多种行为测试（SNAP、横梁行走、足部失误和水迷宫）来评估功能缺损。SNAP在这些测试中独具特色，因为它不需要昂贵的设备，且每只小鼠的测试时间不到5分钟。通过受试者工作特征曲线分析确定，SNAP具有高度的敏感性和特异性。通过科恩kappa方法以及比较不同评分者的敏感性和特异性确定，评分者间的可靠性良好。SNAP在脑损伤小鼠3天时检测到本体感觉、视野和运动强度方面的缺损，并且灵敏到足以检测损伤的程度和恢复情况。然而，随着损伤后时间的变化，各个测试组合成分的作用也有所不同，但每个成分对整体SNAP评分都很重要。SNAP提供了一种灵敏、可靠、省时且经济高效的方法，用于评估单侧脑损伤后小鼠的神经功能缺损。
