Hong Minha, Kim Mia, Kim Tae-Woon, Park Sang-Seo, Kim Myung-Ki, Park Youngja H, Sung Yun-Hee, Shin Mal-Soon
Department of Psychiatry, Myongji Hospital, College of Medicine, Hanyang University, Goyang, Korea.
Department of Cardiovascular Neurologic Disease (Stroke Center), College of Korean Medicine, Kyung Hee University, Seoul, Korea.
Int Neurourol J. 2020 May;24(Suppl 1):S28-38. doi: 10.5213/inj.2040158.079. Epub 2020 May 31.
Thrombotic stroke is a type of ischemic stroke characterized by motor dysfunction and memory impairments. In the present study, the effect of treadmill exercise on motor function and short-term memory was evaluated in relation with synaptic plasticity in the mice with photothrombotic stroke.
Photothrombotic stroke was induced by cortical photothrombotic vascular occlusion. The mice in the treadmill exercise groups performed running on a motorized treadmill for 28 days. Motor function was determined using rota-rod test and foot fault test. Step-through avoidance task was conducted to evaluate short-term memory. Immunohistochemistry for 5-bromo-2'-deoxyuridine and doublecortin was conducted to detect new cell generation. Postsynaptic density protein 95, synaptophysin, brain-derived neurotrophic factor (BDNF), and tyrosine kinase B receptor (TrkB) were determined using western blot. The number of dendritic spines was determined using Golgi stain.
Treadmill exercise improved motor function and short-term memory in mice with the photothrombotic stroke. The infarct size was reduced and the number of dendritic spines and expression of postsynaptic density protein 95 and synaptophysin in the peri-infarct cortex and hippocampus were increased by treadmill exercise in photothrombotic stroke mice. Treadmill exercise enhanced neurogenesis through increasing the expression of the hippocampal BDNF and TrkB in photothrombotic stroke mice.
Treadmill exercise improved motor function and short-term memory through increasing synaptic plasticity and neurogenesis in photothrombotic stroke mice. Treadmill exercise can be used as an effective treatment strategy to improve brain function related to stroke.
血栓性中风是一种缺血性中风，其特征为运动功能障碍和记忆损伤。在本研究中，针对光血栓性中风小鼠，评估了跑步机运动对运动功能和短期记忆的影响，并探讨其与突触可塑性的关系。
通过皮层光血栓性血管闭塞诱导光血栓性中风。跑步机运动组的小鼠在电动跑步机上跑步28天。使用转棒试验和足错误试验测定运动功能。进行避暗任务以评估短期记忆。进行5-溴-2'-脱氧尿苷和双皮质素的免疫组织化学检测以检测新细胞生成。使用蛋白质免疫印迹法测定突触后致密蛋白95、突触素、脑源性神经营养因子（BDNF）和酪氨酸激酶B受体（TrkB）。使用高尔基染色法测定树突棘的数量。
跑步机运动改善了光血栓性中风小鼠的运动功能和短期记忆。在光血栓性中风小鼠中，跑步机运动减少了梗死面积，增加了梗死周围皮层和海马中树突棘的数量以及突触后致密蛋白95和突触素的表达。跑步机运动通过增加光血栓性中风小鼠海马中BDNF和TrkB的表达来增强神经发生。
跑步机运动通过增加光血栓性中风小鼠的突触可塑性和神经发生来改善运动功能和短期记忆。跑步机运动可作为改善与中风相关脑功能的有效治疗策略。
