Wang Chunyan, Yu Bazhang, Knudsen Bernard, Harmon Julie, Moussy Francis, Moussy Yvvone
Department of Chemistry, University of South Florida, 4202 East Fowler Avenue, CHE 205, Tampa, Florida 33620-5250, USA.
Biomacromolecules. 2008 Feb;9(2):561-7. doi: 10.1021/bm701102y. Epub 2008 Jan 1.
Novel hydrogel polymers were prepared, characterized, coated on implantable glucose sensors, and tested in vitro and in vivo. The effects of 2,3-dihydroxypropyl methacrylate (DHPMA) on the swelling, morphology, glass transition (T(g)), and water structure were studied. The results show that the degree of swelling increases with increasing DHPMA content. Scanning electron microscopy (SEM) studies identified uniform, porous structures in samples containing 60-90 mol % DHPMA. Glass-transition temperatures did not change significantly with DHPMA content, but the ratio of freezing to nonfreezing water tended to increase with DHPMA content. Sensors coated with different hydrogels were prepared and in vitro evaluations were performed. The 80% DHPMA hydrogels exhibited optimum sensitivity, response, and stability when coated directly onto the sensor or top of a polyurethane (PU) layer. The histology results show that 80% DHPMA samples exhibit reduced fibrosis and reduced inflammation, resulting in a longer functional life.
制备了新型水凝胶聚合物，对其进行了表征，涂覆在可植入葡萄糖传感器上，并进行了体外和体内测试。研究了甲基丙烯酸2,3 - 二羟丙酯（DHPMA）对溶胀、形态、玻璃化转变温度（T(g)）和水结构的影响。结果表明，溶胀度随DHPMA含量的增加而增大。扫描电子显微镜（SEM）研究确定了含有60 - 90 mol% DHPMA的样品中存在均匀的多孔结构。玻璃化转变温度随DHPMA含量变化不显著，但冷冻水与非冷冻水的比例倾向于随DHPMA含量增加。制备了涂覆不同水凝胶的传感器并进行了体外评估。当直接涂覆在传感器上或聚氨酯（PU）层顶部时，80% DHPMA水凝胶表现出最佳的灵敏度、响应性和稳定性。组织学结果表明，80% DHPMA样品的纤维化和炎症减少，从而延长了功能寿命。
