Taguchi Manabu, Sun Yu-Long, Zhao Chunfeng, Zobitz Mark E, Cha Chung-Ja, Jay Gregory D, An Kai-Nan, Amadio Peter C
Biomechanics Laboratory, Mayo Clinic College of Medicine, 200 First Street S.W., Rochester, MN 55905, USA.
J Bone Joint Surg Am. 2008 Jan;90(1):129-35. doi: 10.2106/JBJS.G.00045.
Lubricin is the principal lubricant in synovial fluid. Although lubricin has been identified in tendons, especially on the surface of intrasynovial tendons such as the flexor digitorum profundus tendon, its ability to improve tendon gliding is unknown. The purpose of this study was to investigate the effects of exogenously applied lubricin on the gliding of extrasynovial tendons in a canine model in vitro.
Forty peroneus longus tendons, along with the proximal pulley in the ipsilateral hind paw, were harvested from adult mongrel dogs. After the gliding resistance of the normal tendons was measured, the tendons were treated with one of the following solutions: saline solution, lubricin, carbodiimide derivatized gelatin (cd-gelatin), carbodiimide derivatized gelatin with hyaluronic acid (cd-HA-gelatin), or carbodiimide derivatized gelatin to which lubricin had been added in a second step (cd-gelatin plus lubricin). Tendon gliding resistance was measured for 1000 cycles of simulated flexion-extension motion of the tendon. Transverse sections of the tendons were examined qualitatively at 100x magnification to estimate surface smoothness after 1000 cycles.
There was no significant difference in the gliding resistance between the tendons treated with saline solution and those treated with lubricin alone, or between the tendons treated with cd-HA-gelatin and those treated with cd-gelatin plus lubricin; however, the gliding resistance of the tendons treated with cd-gelatin plus lubricin was significantly lower than that of the tendons treated with saline solution, lubricin alone, or cd-gelatin alone (p < 0.05). After 1000 cycles of tendon motion, the gliding resistance of the tendons treated with cd-gelatin plus lubricin decreased 18.7% compared with the resistance before treatment, whereas the gliding resistance of the saline-solution-treated controls increased >400%. The tendon surfaces treated with cd-gelatin plus lubricin or with cd-HA-gelatin appeared smooth even after 1000 cycles of tendon motion, whereas the other surfaces appeared roughened.
While the addition of lubricin alone did not affect friction in this tendon gliding model, the results indicate that lubricin may preferentially adhere to a tendon surface pretreated with cd-gelatin and, when so fixed in place, lubricin does have an important effect on tendon lubrication.
润滑素是滑液中的主要润滑剂。尽管在肌腱中已发现润滑素，尤其是在滑膜内肌腱表面，如指深屈肌腱表面，但它改善肌腱滑动的能力尚不清楚。本研究的目的是在体外犬模型中研究外源性应用润滑素对滑膜外肌腱滑动的影响。
从成年杂种犬中采集40条腓骨长肌腱及其同侧后爪的近端滑车。在测量正常肌腱的滑动阻力后，将肌腱用以下溶液之一处理：生理盐水溶液、润滑素、碳二亚胺衍生化明胶（cd-明胶）、含透明质酸的碳二亚胺衍生化明胶（cd-HA-明胶）或第二步添加了润滑素的碳二亚胺衍生化明胶（cd-明胶加润滑素）。对肌腱进行1000次模拟屈伸运动循环，测量其滑动阻力。在100倍放大倍数下对肌腱横切片进行定性检查，以评估1000次循环后表面光滑度。
用生理盐水溶液处理的肌腱与单独用润滑素处理的肌腱之间，以及用cd-HA-明胶处理的肌腱与用cd-明胶加润滑素处理的肌腱之间，滑动阻力无显著差异；然而，用cd-明胶加润滑素处理的肌腱的滑动阻力显著低于用生理盐水溶液、单独润滑素或单独cd-明胶处理的肌腱（p < 0.05）。在肌腱运动1000次循环后，用cd-明胶加润滑素处理的肌腱的滑动阻力与处理前相比降低了18.7%，而用生理盐水溶液处理的对照组的滑动阻力增加了>400%。即使在肌腱运动1000次循环后，用cd-明胶加润滑素或cd-HA-明胶处理的肌腱表面看起来仍很光滑，而其他表面则显得粗糙。
虽然在该肌腱滑动模型中单独添加润滑素不影响摩擦力，但结果表明润滑素可能优先粘附于用cd-明胶预处理的肌腱表面，并且当如此固定到位时，润滑素对肌腱润滑确实有重要作用。
