Yokoyama M, Hinode D, Yoshioka M, Fukui M, Tanabe S, Grenier D, Ito H-O
Department of Preventive Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, and Clinic of Dental Hygiene Section, Tokushima University Medical and Dental Hospital, Tokushima, Japan.
Oral Microbiol Immunol. 2008 Feb;23(1):55-9. doi: 10.1111/j.1399-302X.2007.00391.x.
In a previous study, we showed that the growth of Campylobacter rectus is stimulated by the presence of female sex hormones in the culture medium. In the present study, we examined the relationship between C. rectus levels in the saliva and the periodontal status of pregnant women.
Unstimulated whole saliva was collected from 22 pregnant and 15 non-pregnant women. Periodontal pocket depth (PD) and bleeding on probing (BOP) were recorded. A quantitative real-time polymerase chain reaction was performed to determine the concentrations of suspected periodontopathogenic bacteria in the saliva samples. In addition, the concentration of estradiol in the saliva samples was measured by enzyme immunoassay.
The average age, number of teeth, and total number of bacteria in the saliva of subjects in both groups were similar. The percentage of sites with a PD = 4 mm and the salivary estradiol concentrations were significantly higher in pregnant women than in non-pregnant women. In addition, the percentage of BOP sites and the C. rectus levels in the saliva of the pregnant women tended to be higher than in non-pregnant women, although these differences were not statistically significant. There were positive correlations between C. rectus levels and estradiol concentrations, and between C. rectus levels and the percentage of sites with PD = 4 mm in the pregnant women.
These results indicate that C. rectus levels are higher in the oral flora of pregnant women and that this may be associated with increased salivary estradiol concentrations. This may contribute to periodontal disease progression during pregnancy.
在之前的一项研究中，我们发现培养基中存在女性性激素会刺激直肠弯曲菌的生长。在本研究中，我们检测了孕妇唾液中直肠弯曲菌水平与牙周状况之间的关系。
收集22名孕妇和15名非孕妇的非刺激性全唾液。记录牙周袋深度（PD）和探诊出血（BOP）情况。采用定量实时聚合酶链反应来测定唾液样本中可疑牙周病原菌的浓度。此外，通过酶免疫测定法测量唾液样本中雌二醇的浓度。
两组受试者的平均年龄、牙齿数量和唾液中细菌总数相似。孕妇中PD = 4 mm的部位百分比和唾液雌二醇浓度显著高于非孕妇。此外，孕妇唾液中BOP部位百分比和直肠弯曲菌水平虽高于非孕妇，但差异无统计学意义。孕妇中直肠弯曲菌水平与雌二醇浓度之间以及直肠弯曲菌水平与PD = 4 mm的部位百分比之间存在正相关。
这些结果表明孕妇口腔菌群中直肠弯曲菌水平较高，这可能与唾液雌二醇浓度升高有关。这可能导致孕期牙周疾病进展。
