Young Chiu-Chung, Chou Jui-Hsing, Arun A B, Yen Wen-Shao, Sheu Shih-Yi, Shen Fo-Ting, Lai Wai-An, Rekha P D, Chen Wen-Ming
Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):251-6. doi: 10.1099/ijs.0.65277-0.
A bacterial strain designated YY287(T), isolated from food waste compost, was investigated by polyphasic taxonomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses using the 16S rRNA gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Comamonas; its closest neighbours were the type strains Comamonas testosteroni DSM 50244(T) (96.5%), Comamonas terrigena DSM 7099(T) (95.4%), Comamonas odontotermitis Dant 3-8(T) (95.2%) and Comamonas koreensis KCTC 12005(T) (94.6%). Strain YY287(T) was clearly distinguished from all of these strains using phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics. The major fatty acids were 16:0 (33%), 18:1omega7c (13%) and summed feature 3 (16:1omega7c and/or 15:0 iso 2-OH; 41%). The DNA G+C content of the genomic DNA was 62.8 mol%. It is evident from the genotypic and phenotypic data that strain YY287(T) represents a novel species in the genus Comamonas, for which the name Comamonas composti sp. nov. is proposed. The type strain is YY287(T) (=BCRC 17659(T)=LMG 24008(T)).
从食物垃圾堆肥中分离出的一株名为YY287(T)的细菌菌株，采用多相分类法进行了研究。细胞呈杆状，革兰氏阴性，无色素，不形成芽孢，不发酵。使用16S rRNA基因序列进行的系统发育分析表明，该菌株在丛毛单胞菌属所占据的进化辐射外围形成了一个单系分支；其最接近的亲缘种是睾丸酮丛毛单胞菌DSM 50244(T)（96.5%）、土生丛毛单胞菌DSM 7099(T)（95.4%）、白蚁丛毛单胞菌Dant 3-8(T)（95.2%）和韩国丛毛单胞菌KCTC 12005(T)（94.6%）。通过系统发育分析、DNA-DNA杂交、脂肪酸组成数据以及一系列生理生化特征，菌株YY287(T)与所有这些菌株明显区分开来。主要脂肪酸为16:0（33%）、18:1ω7c（13%）和总和特征3（16:1ω7c和/或15:0异2-OH；41%）。基因组DNA的G+C含量为62.8 mol%。从基因型和表型数据可以明显看出，菌株YY287(T)代表丛毛单胞菌属中的一个新物种，为此提出了新名称堆肥丛毛单胞菌（Comamonas composti sp. nov.）。模式菌株为YY287(T)（=BCRC 17659(T)=LMG 24008(T)）。
