Kim Daniel, Lee Sang-Seob
Department of Bioengineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea.
Curr Microbiol. 2014 Jul;69(1):102-7. doi: 10.1007/s00284-014-0561-4. Epub 2014 Mar 16.
A new bacterial strain, designated as FF42(T), was isolated from feces of domestic pigs-collected from Suwon, Korea-and was characterized to determine its taxonomic position. Strain FF42(T) was observed to be Gram negative, aerobic, non-spore forming, motile, and rod-shaped cells. Based on the phylogenetic and 16S rRNA sequence analyses, it was revealed that strain FF42(T) belonged to the genus Comamonas. The highest degree of sequence similarities was determined to be with Comamonas zonglianii BF-3(T) (96.3 %), Comamonas composti CC-YY287(T) (96.1 %), and Comamonas nitrativorans 23310(T) (95.9 %), while showing less than 95.6 % identity with the remaining Comamonas species. Growth of strain FF42(T) occurred between 25 and 40 °C (optimum, 28 °C) and at pH of 5-9 (optimum, pH 6.0). It grew in the presence of 0-3 % (w/v) NaCl while minimally tolerating at 3 % (w/v) NaCl. Biochemical and physiological tests revealed phenotypic differentiation of strain FF42(T) to other members of the genus Comamonas. The predominant quinone is ubiquinone (Q-8). The major cellular fatty acids were C10:0 3OH, C16:0, summed feature 3 (C16:1 ω7c/C16:1 ω6c), and summed feature 8 (C18:1 ω6c/C18:1 ω7c), all of which have previously been reported to occur in the species of the genus Comamonas. The G+C molar content for strain FF42(T) is 60.2 mol %. Based on phylogenetic and phenotypic analyses, strain FF42(T) (=KEMC 1002-058(T)=JCM 17561(T)) is clearly referred to be a novel species for the genus Comamonas, for which the name Comamonas faecalis sp. nov. is proposed.
从韩国水原采集的家猪粪便中分离出一株新的细菌菌株，命名为FF42(T)，并对其进行了特征鉴定以确定其分类地位。观察到菌株FF42(T)为革兰氏阴性、需氧、不形成芽孢、运动、杆状细胞。基于系统发育和16S rRNA序列分析，发现菌株FF42(T)属于丛毛单胞菌属。确定其与宗联丛毛单胞菌BF-3(T)（96.3%）、堆肥丛毛单胞菌CC-YY287(T)（96.1%）和硝酸盐还原丛毛单胞菌23310(T)（95.9%）的序列相似性最高，而与其余丛毛单胞菌属物种的同一性低于95.6%。菌株FF42(T)在25至40℃（最适温度28℃）和pH值5至9（最适pH值6.0）条件下生长。它在0至3%（w/v）NaCl存在下生长，在3%（w/v）NaCl时耐受性最低。生化和生理测试显示菌株FF42(T)与丛毛单胞菌属其他成员的表型差异。主要醌类为泛醌（Q-8）。主要细胞脂肪酸为C10:0 3OH、C16:0、总和特征3（C16:1 ω7c/C16:1 ω6c）和总和特征8（C18:1 ω6c/C18:1 ω7c），所有这些脂肪酸此前已报道在丛毛单胞菌属物种中出现。菌株FF42(T)的G+C摩尔含量为60.2 mol%。基于系统发育和表型分析，菌株FF42(T)（=KEMC 1002-058(T)=JCM 17561(T)）显然被认为是丛毛单胞菌属的一个新物种，为此提出名为粪丛毛单胞菌（Comamonas faecalis sp. nov.）的名称。
