Miwa Hiroki, Ahmed Iftikhar, Yoon Jaewoo, Yokota Akira, Fujiwara Toru
Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo 113-8657, Japan.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):286-9. doi: 10.1099/ijs.0.65315-0.
A non-spore-forming, Gram-negative, motile, rod-shaped, boron-accumulating bacterium isolated from soil was characterized by polyphasic taxonomy. The strain, designated BAM-48(T), was positive for catalase and oxidase activities and grew at 4-37 degrees C, 0-1% NaCl and pH 5-9. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate BAM-48(T) was closely related to Variovorax paradoxus IAM 12373(T) (99.3% sequence similarity), Variovorax soli GH9-3(T) (98.3%) and Variovorax dokdonensis DS-43(T) (97.0%). DNA-DNA hybridization values between strain BAM-48(T) and V. paradoxus NBRC 15149(T), V. soli KACC 11579(T) and V. dokdonensis KCTC 12544(T) were only 49.1, 25.4 and 24.6%, respectively. The major respiratory quinone was ubiquinone 8 (Q-8). The DNA G+C content was 71.2 mol%. Strain BAM-48(T) contained C(16:0) (36.1%), C(16:1)omega7c (21.4%) and C(17:0) cyclo (19.0%) as the major fatty acids. Based on phenotypic, chemotaxonomic and phylogenetic data, strain BAM-48(T) should be classified as a member of a novel species in the genus Variovorax, for which the name Variovorax boronicumulans sp. nov. is proposed. The type strain is BAM-48(T) (=NBRC 103145(T) =KCTC 22010(T)).
从土壤中分离出的一株非芽孢形成、革兰氏阴性、具运动性、杆状、能积累硼的细菌，通过多相分类法进行了表征。该菌株命名为BAM-48(T)，过氧化氢酶和氧化酶活性呈阳性，在4-37℃、0-1% NaCl和pH 5-9条件下生长。16S rRNA基因序列的比较分析表明，新分离株BAM-48(T)与奇异贪噬菌IAM 12373(T)（序列相似性99.3%）、土壤贪噬菌GH9-3(T)（98.3%）和独岛贪噬菌DS-43(T)（97.0%）密切相关。菌株BAM-48(T)与奇异贪噬菌NBRC 15149(T)、土壤贪噬菌KACC 11579(T)和独岛贪噬菌KCTC 12544(T)之间的DNA-DNA杂交值分别仅为49.1%、25.4%和24.6%。主要呼吸醌为泛醌8（Q-8）。DNA G+C含量为71.2 mol%。菌株BAM-48(T)含有C(16:0)（36.1%）、C(16:1)ω7c（21.4%）和C(17:0)环丙烷（19.0%）作为主要脂肪酸。基于表型、化学分类和系统发育数据，菌株BAM-48(T)应归类为贪噬菌属中的一个新物种，为此提出新名称嗜硼贪噬菌（Variovorax boronicumulans）。模式菌株为BAM-48(T)（=NBRC 103145(T)=KCTC 22010(T)）。
