Jiang Yu-Hang, Yin Ce-Ce, Yang Lei-Lei, Xin Yu-Hua, Liu Qing, Ye Jian
State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Laboratory of Vector-Borne Diseases and Biomanufacturing, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2025 Aug;75(8). doi: 10.1099/ijsem.0.006895.
Thirteen novel strains - J2L1-78, J2P1-59, J22R24, J22R133, J22P168, J22P271, J22R187, J22R193, J2L1-63, J2R1-6, J22G21, J22G47, and J31P216 - were isolated from the rhizosphere of 20-year-old ginseng in Jilin Province, P.R. China. These strains were Gram-stain-negative, aerobic and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequences indicated their affiliation to the genus , with 98.8-99.6% sequence similarity to Gsoil 3165, UCM-2 and UC38. Phylogenomic analysis demonstrated their distinctiveness from closely related species. Average nucleotide identity (ANI) values among these strains confirm their representation of eight distinct species; additionally, ANI and digital DNA-DNA hybridization values between these strains and their closest relatives were below 92.5 and 54.1%, respectively. Eight representative strains contained C, summed feature 3 (C 7 and/or C 6) and C cyclo as major fatty acids. Based on phenotypic, phylogenetic and genotypic data, these 13 strains comprise 8 novel species within the genus , for which we propose the following names: sp. nov. (J2L1-78=CGMCC 1.60704=KACC 23365), sp. nov. (J2P1-59=CGMCC 1.60707=KACC 23366), sp. nov. (J22R24=CGMCC 1.61001=KACC 23367), sp. nov. (J22R133=CGMCC 1.61263=KACC 23368), sp. nov. (J22P168=CGMCC 1.64555=KACC 23372), sp. nov. (J22P271=CGMCC 1.64593=KACC 23373), sp. nov. (J22R187=CGMCC 1.64629=KACC 23374) and sp. nov. (J22R193=CGMCC 1.64631=KACC 23375).
从中国吉林省20年生人参的根际土壤中分离出13株新菌株——J2L1-78、J2P1-59、J22R24、J22R133、J22P168、J22P271、J22R187、J22R193、J2L1-63、J2R1-6、J22G21、J22G47和J31P216。这些菌株革兰氏染色阴性，好氧，呈杆状。基于16S rRNA基因序列的系统发育分析表明它们属于某一属，与Gsoil 3165、UCM-2和UC38的序列相似性为98.8 - 99.6%。系统基因组分析证明了它们与近缘物种的差异。这些菌株之间的平均核苷酸同一性（ANI）值证实它们代表了8个不同的物种；此外，这些菌株与其最亲近的亲缘种之间的ANI值和数字DNA-DNA杂交值分别低于92.5%和54.1%。8株代表性菌株含有C、总和特征3（C7和/或C6）和C环丙烷脂肪酸作为主要脂肪酸。基于表型、系统发育和基因型数据，这13株菌株在该属内构成8个新物种，我们为此提出以下名称：新种（J2L1-78 = CGMCC 1.60704 = KACC 23365）、新种（J2P1-59 = CGMCC 1.60707 = KACC 23366）、新种（J22R24 = CGMCC 1.61001 = KACC 23367）、新种（J22R133 = CGMCC 1.61263 = KACC 23368）、新种（J22P168 = CGMCC 1.64555 = KACC 23372）、新种（J22P271 = CGMCC 1.64593 = KACC 23373）、新种（J22R187 = CGMCC 1.64629 = KACC 23374）和新种（J22R193 = CGMCC 1.64631 = KACC 23375）。
