Gao Jun-lian, Yuan Mei, Wang Xu-ming, Qiu Tian-lei, Li Ji-wei, Liu Hong-can, Li Xiu-ai, Chen Jian, Sun Jian-guang
Beijing Agro- Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 Jan;107(1):65-72. doi: 10.1007/s10482-014-0304-3. Epub 2014 Dec 12.
A 1-aminocyclopropane-1-carboxylate deaminase producing bacterium, designated GXGD002(T), was isolated from the rhizosphere of banana plants cultivated in Guangxi province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXGD002(T) is a member of the genus Variovorax. High levels of 16S rRNA gene sequence similarity are found between strain GXGD002(T) and Variovorax paradoxus DSM 30034(T) (99.4 %), Variovorax ginsengisoli KCTC 12583(T) (99.1 %), Variovorax boronicumulans KCTC 22010(T) (99.0 %), Variovorax soli DSM18216(T) (98.7 %), Variovorax defluvii DSM 27259(T) (98.1 %) and Variovorax dokdonensis KCTC 12544(T) (97.4 %) respectively. However, the DNA-DNA hybridization values between strain GXGD002(T) and its closely related species V. paradoxus DSM 30034(T), V. ginsengisoli KCTC 12583(T) and V. boronicumulans KCTC 22010(T) were found to be 40.7, 30.9 and 23.7 %, respectively. The DNA G + C content of strain GXGD002(T) was found to be 67.8 mol%. The major fatty acids of strain GXGD002(T) are C16:0 (20.3 %), C10:0 3OH (18.4 %), C17:0 cyclo (18.9 %), C18:1w7c (12.3 %) and summed feature 3 (13.9 %). The predominant respiratory quinone was identified as ubiquinone-8 (Q-8) and the major polar lipids as phosphatidylethanolamine and phosphatidylglycerol. The results of polyphasic taxonomic study including physiological and biochemical tests, whole-cell SDS-PAGE profiles and chemotaxonomic analysis allowed a clear differentiation of strain GXGD002(T) from the other species in the genus Variovorax. Based on these results, a new species, Variovorax guangxiensis, is proposed. The type strain is GXGD002(T) (=DSM 27352(T) = ACCC 05911(T)).
从中国广西种植的香蕉植株根际分离出一株产1-氨基环丙烷-1-羧酸脱氨酶的细菌，命名为GXGD002(T)。基于16S rRNA基因序列的系统发育分析表明，菌株GXGD002(T)是贪噬菌属的一个成员。菌株GXGD002(T)与嗜麦芽寡养单胞菌DSM 30034(T)（99.4%）、人参贪噬菌KCTC 12583(T)（99.1%）、硼酸贪噬菌KCTC 22010(T)（99.0%）、土壤贪噬菌DSM18216(T)（98.7%）、污水贪噬菌DSM 27259(T)（98.1%）和独岛贪噬菌KCTC 12544(T)（97.4%）的16S rRNA基因序列相似性分别较高。然而，菌株GXGD002(T)与其近缘种嗜麦芽寡养单胞菌DSM 30034(T)、人参贪噬菌KCTC 12583(T)和硼酸贪噬菌KCTC 22010(T)之间的DNA-DNA杂交值分别为40.7%、30.9%和23.7%。菌株GXGD002(T)的DNA G+C含量为67.8 mol%。菌株GXGD002(T)的主要脂肪酸为C16:0（20.3%）、C10:0 3OH（18.4%）、C17:0环丙烷（18.9%）、C18:1w7c（12.3%）和总和特征3（13.9%）。主要呼吸醌被鉴定为泛醌-8（Q-8），主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。包括生理生化试验、全细胞SDS-PAGE图谱和化学分类分析在内的多相分类研究结果使菌株GXGD002(T)能够与贪噬菌属的其他物种明显区分开来。基于这些结果，提出了一个新物种，即广西贪噬菌。模式菌株为GXGD002(T)（=DSM 27352(T)=ACCC 05911(T)）。
