Hu Jiang-Ning, Zhu Xue-Mei, Han Li-Kun, Saito Masato, Sun Yin-Shi, Yoshikawa Masayuki, Kimura Yoshiyuki, Zheng Yi-Nan
Department of Food Science and Technology, Chungnam National University, Republic of Korea.
Chem Pharm Bull (Tokyo). 2008 Jan;56(1):12-6. doi: 10.1248/cpb.56.12.
To investigate the anti-obesity effects of escins extracted from the seeds of Aesculus turbinata BLUME, anti-obesity models in vitro and in vivo were employed. In a preliminary experiment, different solvent fractions of Aesculus turbinata BlUME as well as two isolated compounds were tested for their effects on pancreatic lipase (PL) in vitro. Subsequently, female ICR mice were fed a high fat diet with or without different concentrations of total escins for 11 weeks to examine body weight, parametrial adipose tissue weight, and hepatic triacylglycerol (TG) and total cholesterol (TC) contents. Plasma triacylglycerol levels (TG) after oral administration of lipid emulsions to rats were also investigated. The results showed that total escins (1 mg/ml) as well as two compounds isolated from total escins, namely escin Ib and IIa, showed inhibitory effects on PL activity. In vivo, total escins suppressed the increase in body weight, parametrial adipose tissue weight, TG content, and TC content in mice's liver; TG content in rat plasma was also reduced at 1, 2 and 3 h after oral administration of the lipid emulsion plus different concentrations of escins compared to those in the lipid emulsion groups. Meanwhile, mice fed a high fat diet plus 2% total escins for 3 d had an increased TG level in the feces compared to the HF group. The reason for this may be due to a delay in the intestinal absorption of dietary fat by inhibiting PL activity.
为研究从日本七叶树种子中提取的七叶皂苷的抗肥胖作用，采用了体外和体内抗肥胖模型。在初步实验中，测试了日本七叶树不同溶剂提取物以及两种分离化合物对体外胰脂肪酶（PL）的影响。随后，给雌性ICR小鼠喂食含或不含不同浓度总七叶皂苷的高脂肪饮食11周，以检测体重、子宫旁脂肪组织重量以及肝脏三酰甘油（TG）和总胆固醇（TC）含量。还研究了给大鼠口服脂质乳剂后血浆三酰甘油水平（TG）。结果表明，总七叶皂苷（1 mg/ml）以及从总七叶皂苷中分离出的两种化合物，即七叶皂苷Ib和IIa，对PL活性具有抑制作用。在体内，总七叶皂苷抑制了小鼠体重、子宫旁脂肪组织重量、TG含量以及肝脏TC含量的增加；与脂质乳剂组相比，口服脂质乳剂加不同浓度七叶皂苷后1、2和3小时，大鼠血浆中的TG含量也有所降低。同时，与高脂肪饮食组相比，喂食含2%总七叶皂苷的高脂肪饮食3天的小鼠粪便中的TG水平升高。其原因可能是通过抑制PL活性延缓了膳食脂肪的肠道吸收。
