Nam Hyeran, Whang Kyunghee, Lee Yeonhee
Culture Collection of Antimicrobial Resistant Microbes, Department of Biology, Seoul Women's University, Seoul, Republic of Korea.
J Microbiol. 2007 Dec;45(6):515-20.
Vaginal lactic acid-producing bacteria of 80 pre-menopausal women were studied by isolation on Blood and DeMan-Rogosa-Sharpe agar, PCR with group-specific primers for Lactobacillus-denaturing gradient gel electrophoresis (DGGE), and PCR with specific primers for V3 region in 16S rRNA-temporal temperature gel electrophoresis (TTGE). Conventional isolation method on media detected only one lactobacillus (Lactobacillus brevis) while TTGE detected only Lactobacillus sp. DGGE detected seven Lactobacillus species; L. coleohominis, L. crispatus, L. iners, L. reuteri, L. rhamnosus, L. vaginalis, and Leuconostoc lactis. L. acidophilus and L. gasseri, which are prevalent in Western women, were not detected in Korean women. Furthermore, L. rhamnosus, Leuc. lactis, L. coleohominis, and Weissella cibaria, which were not previously reported in the vaginal microbiota of Korean women, were detected. The five most prevalent LABs in vaginal microbiota in Korean women were L. iners, Enterococcus faecalis, L. crispatus, Leuc. lactis, and W. cibaria.
通过在血琼脂和德氏乳杆菌-罗格斯-夏普琼脂上进行分离、使用针对乳酸杆菌的组特异性引物进行聚合酶链反应-变性梯度凝胶电泳（DGGE）以及使用针对16S核糖体RNA V3区域的特异性引物进行聚合酶链反应-时间温度凝胶电泳（TTGE），对80名绝经前女性的阴道产乳酸细菌进行了研究。传统的培养基分离方法仅检测到一种乳酸杆菌（短乳杆菌），而TTGE仅检测到乳酸杆菌属。DGGE检测到七种乳酸杆菌；即科氏乳杆菌、卷曲乳杆菌、惰性乳杆菌、罗伊氏乳杆菌、鼠李糖乳杆菌、阴道乳杆菌和乳酸明串珠菌。在西方女性中普遍存在的嗜酸乳杆菌和加氏乳杆菌在韩国女性中未被检测到。此外，还检测到了韩国女性阴道微生物群中先前未报道的鼠李糖乳杆菌、乳酸明串珠菌、科氏乳杆菌和食窦魏斯氏菌。韩国女性阴道微生物群中最常见的五种乳酸菌是惰性乳杆菌、粪肠球菌、卷曲乳杆菌、乳酸明串珠菌和食窦魏斯氏菌。
