Wang Q, Dickson G R, Abram W P, Carr K E
School of Biomedical Science/Anatomy, Queen's University of Belfast, Northern Ireland, U.K.
Br J Dermatol. 1994 May;130(5):551-60. doi: 10.1111/j.1365-2133.1994.tb13098.x.
To date, there have been few morphological investigations of the effect of electron radiation on the healing of skin wounds in rats. The present morphological study examines the wound repair process in electron-irradiated rat skin by electron microscopy. Standardized, full-thickness, incisional wounds were made in the lower dorsal skin of animals which had been locally irradiated with 9.6 Gy electron radiation 7 days previously. The irradiation dose was maximal at 3 mm depth. Twenty-four rats were used in the investigation; 12 were irradiated and 12 sham-irradiated. Three rats from each experimental group were killed at 1, 3, 7 and 14-day time intervals after wounding. The morphological effect of electron irradiation on the repair of each wound was investigated by light microscopy (LM) and scanning electron microscopy (SEM). New granulation tissue visualized by SEM was quantified using computerized image analysis. The results suggest that a single, partial-body, controlled depth dose of electron irradiation delays wound repair. LM showed that there is a depression of the inflammatory cell and tissue exudate response, slowing of epithelial migration, and a decrease in fibroblast representation, together with a delay in the formation of collagen bundles. Granulation tissue formation was impaired up to 7 days post-wounding, but was restored to around control values by day 14, indicating that healing was delayed. However, as the healing of normal tissue was not prevented, this study supports a preoperative role for the use of low-dose electron irradiation therapy for the treatment of electron-sensitive superficial pathologies in surgical practice.
迄今为止，关于电子辐射对大鼠皮肤伤口愈合影响的形态学研究较少。本形态学研究通过电子显微镜检查电子辐照大鼠皮肤的伤口修复过程。在7天前局部接受9.6 Gy电子辐射的动物的背部下侧皮肤制作标准化的全层切开伤口。辐照剂量在3 mm深度处最大。本研究使用了24只大鼠；12只接受辐照，12只接受假辐照。在受伤后的1、3、7和14天时间间隔，从每个实验组处死3只大鼠。通过光学显微镜（LM）和扫描电子显微镜（SEM）研究电子辐照对每个伤口修复的形态学影响。使用计算机图像分析对通过SEM观察到的新肉芽组织进行定量。结果表明，单次、局部身体、可控深度剂量的电子辐照会延迟伤口修复。光学显微镜显示炎症细胞和组织渗出反应受到抑制，上皮迁移减慢，成纤维细胞数量减少，同时胶原束形成延迟。伤口愈合后7天内肉芽组织形成受损，但到第14天恢复到接近对照值，表明愈合延迟。然而，由于正常组织的愈合未被阻止，本研究支持在外科实践中使用低剂量电子辐照疗法治疗对电子敏感的浅表病变的术前作用。
