Wang Ning, Li Peibo, Wang Yonggang, Peng Wei, Wu Zhong, Tan Suiyi, Liang Shaoling, Shen Xiao, Su WeiWei
Guangzhou Quality R&D Center of Traditional Chinese Medicine, School of Life Science, Sun Yat-sen University, Guangzhou 510275, PR China.
J Ethnopharmacol. 2008 Feb 28;116(1):1-6. doi: 10.1016/j.jep.2007.08.031. Epub 2007 Aug 19.
To investigate the hepatoprotective activity of different parts of Hypericum japonicum against carbon tetrachloride(CCl(4))-induced hepatitis and alpha-naphthyl-isothiocyanate (ANIT)-induced cholestasis.
Mice were divided into groups and then administrated orally with solutions extracted from herbs before they were modeled in the experiments. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (T-BIL) in serum were evaluated. HPLC fingerprint was used for phytochemical analysis of the extracts.
The total aqueous extract of Hypericum japonicum had an obvious effect on the decreasing of AST, ALT and T-BIL levels in serum. The isolated fraction IV (F4) exhibited a preferable activity of ameliorating cholestasis, while Fraction V (F5) was more efficacious in protecting liver from injury. Chemical fingerprint indicated that F5 contained several flavonoids which might be the active chemicals against hepatotoxicity.
Different fractions of Hypericum japonicum manifest different effect, indicating their different potentials as candidacies of new drugs.
研究地耳草不同部位对四氯化碳（CCl₄）诱导的肝炎和α-萘异硫氰酸酯（ANIT）诱导的胆汁淤积的保肝活性。
将小鼠分组，在实验建模前口服草药提取物溶液。评估血清中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和总胆红素（T-BIL）水平。采用高效液相色谱指纹图谱对提取物进行植物化学分析。
地耳草总水提取物对降低血清中AST、ALT和T-BIL水平有明显作用。分离得到的组分IV（F4）表现出较好的改善胆汁淤积活性，而组分V（F5）在保护肝脏免受损伤方面更有效。化学指纹图谱表明F5含有几种黄酮类化合物，可能是抗肝毒性的活性成分。
地耳草不同组分表现出不同的作用，表明它们作为新药候选物具有不同的潜力。
