Harwood Colin R, Cranenburgh Rocky
Institute of Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK.
Trends Microbiol. 2008 Feb;16(2):73-9. doi: 10.1016/j.tim.2007.12.001. Epub 2008 Jan 7.
Bacillus subtilis and its close relatives are widely used for the production of enzymes for the detergent, food and beverage industries. These organisms not only produce an appropriate range of enzymes but also have the capacity to secrete them into the culture medium at high concentrations. Purification from the culture medium rather than from the cytoplasm considerably reduces downstream processing costs. In recent years, considerable effort has been aimed at developing B. subtilis as a host for the production of heterologous proteins. The folded state of the target protein at various stages of the secretion pathway has proved to be important.
枯草芽孢杆菌及其近缘菌被广泛用于生产洗涤剂、食品和饮料工业所需的酶。这些微生物不仅能产生一系列合适的酶，还具备将它们高浓度分泌到培养基中的能力。从培养基而非细胞质中进行纯化，可大幅降低下游加工成本。近年来，人们付出了巨大努力，致力于将枯草芽孢杆菌开发成生产异源蛋白的宿主。事实证明，目标蛋白在分泌途径各个阶段的折叠状态至关重要。
