Olesen Pelle Thonning, Olsen Anja, Frandsen Henrik, Frederiksen Kirsten, Overvad Kim, Tjønneland Anne
National Food Institute, Technical University of Denmark, Søborg, Denmark.
Int J Cancer. 2008 May 1;122(9):2094-100. doi: 10.1002/ijc.23359.
Acrylamide, a probable human carcinogen, is formed in several foods during high-temperature processing. So far, epidemiological studies have not shown any association between human cancer risk and dietary exposure to acrylamide. The purpose of this study was to conduct a nested case control study within a prospective cohort study on the association between breast cancer and exposure to acrylamide using biomarkers. N-terminal hemoglobin adduct levels of acrylamide and its genotoxic metabolite, glycidamide in red blood cells were analyzed (by LC/MS/MS) as biomarkers of exposure on 374 breast cancer cases and 374 controls from a cohort of postmenopausal women. The adduct levels of acrylamide and glycidamide were similar in cases and controls, with smokers having much higher levels (approximately 3 times) than nonsmokers. No association was seen between acrylamide-hemoglobin levels and breast cancer risk neither unadjusted nor adjusted for the potential confounders HRT duration, parity, BMI, alcohol intake and education. After adjustment for smoking behavior, however, a positive association was seen between acrylamide-hemoglobin levels and estrogen receptor positive breast cancer with an estimated incidence rate ratio (95% CI) of 2.7 (1.1-6.6) per 10-fold increase in acrylamide-hemoglobin level. A weak association between glycidamide hemoglobin levels and incidence of estrogen receptor positive breast cancer was also found, this association, however, entirely disappeared when acrylamide and glycidamide hemoglobin levels were mutually adjusted.
丙烯酰胺是一种可能的人类致癌物，在多种食品的高温加工过程中形成。到目前为止，流行病学研究尚未显示人类癌症风险与膳食中丙烯酰胺暴露之间存在任何关联。本研究的目的是在前瞻性队列研究中进行一项巢式病例对照研究，以使用生物标志物探讨乳腺癌与丙烯酰胺暴露之间的关联。对来自绝经后女性队列的374例乳腺癌病例和374例对照，分析了红细胞中丙烯酰胺及其遗传毒性代谢物环氧丙酰胺的N端血红蛋白加合物水平（通过液相色谱/串联质谱法）作为暴露生物标志物。病例组和对照组中丙烯酰胺和环氧丙酰胺的加合物水平相似，吸烟者的水平（约为3倍）远高于非吸烟者。无论是否对潜在混杂因素激素替代疗法持续时间、产次、体重指数、酒精摄入量和教育程度进行调整，丙烯酰胺-血红蛋白水平与乳腺癌风险之间均未发现关联。然而，在对吸烟行为进行调整后，丙烯酰胺-血红蛋白水平与雌激素受体阳性乳腺癌之间存在正相关，丙烯酰胺-血红蛋白水平每增加10倍，估计发病率比（95%可信区间）为2.7（1.1 - 6.6）。还发现环氧丙酰胺血红蛋白水平与雌激素受体阳性乳腺癌发病率之间存在弱关联，然而，当对丙烯酰胺和环氧丙酰胺血红蛋白水平进行相互调整时，这种关联完全消失。
