Department of Diet, Cancer and Health, Institute of Cancer Epidemiology, Danish Cancer Society, Strandboulevarden 49, Copenhagen, Denmark.
Br J Nutr. 2011 May;105(9):1381-7. doi: 10.1017/S0007114510005003. Epub 2011 Jan 28.
Acrylamide (AA) is a probable human carcinogen that is formed in heat-treated carbohydrate-rich foods. The validity of FFQ to assess AA exposure has been questioned. The aim of the present cross-sectional study was to investigate dietary determinants of Hb-AA and Hb-glycidamide (GA) adducts. The study included 537 non-smoking women aged 50-65 years who participated in the Diet, Cancer and Health cohort (1993-97). At study baseline, blood samples and information on dietary and lifestyle variables obtained from self-administered questionnaires were collected. From blood samples, Hb-AA and Hb-GA in erythrocytes were analysed by liquid chromatography/MS/MS. Dietary determinants were evaluated by multiple linear regression analyses adjusted for age and smoking behaviour among ex-smokers. The median for Hb-AA was 35 pmol/g globin (5th percentile 17, 95th percentile 89) and for Hb-GA 21 pmol/g globin (5th percentile 8, 95th percentile 49). Of the dietary factors studied, intakes of coffee and chips were statistically significantly associated with a 4 % per 200 g/d (95 % CI 2, 7; P < 0·0001) and an 18 % per 5 g/d (95 % CI 6, 31; P = 0·002) higher Hb-AA, respectively. This model explained 17 % of the variation in Hb-AA. Intakes of coffee and biscuits/crackers were statistically significantly associated with a 3 % per 200 g/d (95 % CI 1, 6; P = 0·005) and 12 % per 10 g/d (95 % CI 3, 23; P = 0·01) higher Hb-GA, respectively. This model explained 12 % of the variation in Hb-GA. In conclusion, only a few dietary determinants of Hb-AA and Hb-GA were identified. Thus, the present study implies that dietary intake measured by an FFQ explains only to a limited extent the variation in Hb-AA and Hb-GA concentrations.
丙烯酰胺（AA）是一种可能的人类致癌物质，存在于高温处理的富含碳水化合物的食物中。 问卷式食物频率表（FFQ）评估 AA 暴露的有效性受到了质疑。 本横断面研究的目的是调查 Hb-AA 和 Hb-丙烯醛（GA）加合物的饮食决定因素。 该研究包括 1993-97 年参加饮食、癌症和健康队列的 537 名 50-65 岁的不吸烟女性。 在研究基线时，采集血样并通过自填问卷收集饮食和生活方式变量信息。 从血样中，通过液相色谱/质谱联用仪分析红细胞中的 Hb-AA 和 Hb-GA。 通过多元线性回归分析，调整了非吸烟者的年龄和吸烟行为，评估了饮食决定因素。 Hb-AA 的中位数为 35 pmol/g 球蛋白（第 5 百分位数 17，第 95 百分位数 89），Hb-GA 为 21 pmol/g 球蛋白（第 5 百分位数 8，第 95 百分位数 49）。 在研究的饮食因素中，咖啡和薯片的摄入量分别与 Hb-AA 每增加 200g/d（95%CI 2，7；P<0.0001）和每增加 5g/d（95%CI 6，31；P=0.002）呈统计学显著正相关。 该模型解释了 Hb-AA 变异的 17%。 咖啡和饼干/薄脆饼干的摄入量分别与 Hb-AA 每增加 200g/d（95%CI 1，6；P=0.005）和每增加 10g/d（95%CI 3，23；P=0.01）呈统计学显著正相关。 该模型解释了 Hb-GA 变异的 12%。 总之，仅确定了 Hb-AA 和 Hb-GA 的少数饮食决定因素。 因此，本研究表明，通过 FFQ 测量的饮食摄入仅在有限程度上解释了 Hb-AA 和 Hb-GA 浓度的变化。
