Obón-Santacana Mireia, Lujan-Barroso Leila, Freisling Heinz, Cadeau Claire, Fagherazzi Guy, Boutron-Ruault Marie-Christine, Kaaks Rudolf, Fortner Renée T, Boeing Heiner, Ramón Quirós J, Molina-Montes Esther, Chamosa Saioa, Castaño José María Huerta, Ardanaz Eva, Khaw Kay-Tee, Wareham Nick, Key Tim, Trichopoulou Antonia, Lagiou Pagona, Naska Androniki, Palli Domenico, Grioni Sara, Tumino Rosario, Vineis Paolo, De Magistris Maria Santucci, Bueno-de-Mesquita H B, Peeters Petra H, Wennberg Maria, Bergdahl Ingvar A, Vesper Hubert, Riboli Elio, Duell Eric J
Unit of Nutrition and Cancer, Cancer Epidemiology Research Program, Catalan Institute of Oncology, Bellvitge Biomedical Research Institute (ICO-IDIBELL), Avda Gran Via Barcelona 199-203, L'Hospitalet de Llobregat, 08908, Barcelona, Spain.
Dietary Exposure Assessment Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372, Lyon, France.
Eur J Nutr. 2017 Apr;56(3):1157-1168. doi: 10.1007/s00394-016-1165-5. Epub 2016 Feb 5.
Acrylamide was classified as 'probably carcinogenic' to humans in 1994 by the International Agency for Research on Cancer. In 2002, public health concern increased when acrylamide was identified in starchy, plant-based foods, processed at high temperatures. The purpose of this study was to identify which food groups and lifestyle variables were determinants of hemoglobin adduct concentrations of acrylamide (HbAA) and glycidamide (HbGA) in 801 non-smoking postmenopausal women from eight countries in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort.
Biomarkers of internal exposure were measured in red blood cells (collected at baseline) by high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) . In this cross-sectional analysis, four dependent variables were evaluated: HbAA, HbGA, sum of total adducts (HbAA + HbGA), and their ratio (HbGA/HbAA). Simple and multiple regression analyses were used to identify determinants of the four outcome variables. All dependent variables (except HbGA/HbAA) and all independent variables were log-transformed (log) to improve normality. Median (25th-75th percentile) HbAA and HbGA adduct levels were 41.3 (32.8-53.1) pmol/g Hb and 34.2 (25.4-46.9) pmol/g Hb, respectively.
The main food group determinants of HbAA, HbGA, and HbAA + HbGA were biscuits, crackers, and dry cakes. Alcohol intake and body mass index were identified as the principal determinants of HbGA/HbAA. The total percent variation in HbAA, HbGA, HbAA + HbGA, and HbGA/HbAA explained in this study was 30, 26, 29, and 13 %, respectively.
Dietary and lifestyle factors explain a moderate proportion of acrylamide adduct variation in non-smoking postmenopausal women from the EPIC cohort.
1994年，国际癌症研究机构将丙烯酰胺列为对人类“可能致癌”物质。2002年，当在高温加工的含淀粉植物性食品中发现丙烯酰胺时，公众对其健康的担忧加剧。本研究的目的是确定在欧洲癌症与营养前瞻性调查（EPIC）队列中，来自八个国家的801名非吸烟绝经后女性中，哪些食物组和生活方式变量是丙烯酰胺血红蛋白加合物浓度（HbAA）和环氧丙酰胺血红蛋白加合物浓度（HbGA）的决定因素。
通过高效液相色谱/串联质谱法（HPLC/MS/MS）在红细胞（基线时采集）中测量内暴露生物标志物。在这项横断面分析中，评估了四个因变量：HbAA、HbGA、总加合物总和（HbAA + HbGA）及其比值（HbGA/HbAA）。采用简单和多元回归分析来确定四个结果变量的决定因素。所有因变量（HbGA/HbAA除外）和所有自变量均进行对数转换（log）以改善正态性。HbAA和HbGA加合物水平的中位数（第25 - 75百分位数）分别为41.3（32.8 - 53.1）pmol/g Hb和34.2（25.4 - 46.9）pmol/g Hb。
HbAA、HbGA和HbAA + HbGA的主要食物组决定因素是饼干、薄脆饼干和干蛋糕。酒精摄入量和体重指数被确定为HbGA/HbAA的主要决定因素。本研究中解释的HbAA、HbGA、HbAA + HbGA和HbGA/HbAA的总变异百分比分别为30%、26%、29%和13%。
饮食和生活方式因素在EPIC队列的非吸烟绝经后女性中解释了丙烯酰胺加合物变异的适度比例。
