Kamiya H, Liu P H, Ishigooka S, Blas B L
Department of Parasitology, Hirosaki University School of Medicine, Aomori, Japan.
Southeast Asian J Trop Med Public Health. 1991 Sep;22(3):417-21.
Antigenicity of Schistosoma mansoni and S. japonicum eggs preserved in ethanol or acetone were assessed in a circumoval precipitin (COP) assay. The egg antigens were found to retain sufficiently their COP reactivity for the diagnosis of both schistosomiasis mansoni and japonica, although their reactivity became lower than that of lyophilized eggs. These alternative preparations for COP tests have advantages, such as keeping eggs directly in fixatives soon after the egg-purification process. Furthermore, evaporation-process may cause eggshell cleavages which facilitate the reaction. The possible usefulness of those eggs in COP assays in local endemic areas is discussed.
在环卵沉淀试验（COP）中评估了保存在乙醇或丙酮中的曼氏血吸虫和日本血吸虫虫卵的抗原性。发现这些虫卵抗原在曼氏血吸虫病和日本血吸虫病的诊断中仍充分保留其COP反应性，尽管其反应性低于冻干虫卵。这些用于COP试验的替代制剂具有一些优点，例如在虫卵纯化后可将虫卵直接保存在固定剂中。此外，蒸发过程可能导致卵壳破裂，从而促进反应。文中讨论了这些虫卵在当地流行地区的COP试验中的潜在用途。
