Chiemsombat Pissawan, Gajanandana Oraprapai, Warin Nuchanard, Hongprayoon Ratchanee, Bhunchoth Anjana, Pongsapich Preyapan
Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom, 73140, Thailand.
Arch Virol. 2008;153(3):571-7. doi: 10.1007/s00705-007-0024-3. Epub 2008 Jan 10.
Twenty-eight isolates of tospoviruses associated with tomato, pepper, cucurbits, peanut, and Physalis plants collected from fields in different regions of Thailand were characterized. On the basis of N gene and protein sequence relationships, three tospoviruses were identified, namely Watermelon silver mottle virus (WSMoV), Capsicum chlorosis virus (CaCV), and Melon yellow spot virus (MYSV). CLUSTAL analysis of selected N protein sequences showed different isolates of CaCV in three distinct clades. Based on necrosis symptoms on tomato and their 93% identity to CaCV isolates in the other two clades, CaCV-TD8, CaCV-AIT and CaCV-KS16-Thailand tomato tospovirus were designated as CaCV-tomato necrosis strain. A phylogenetic tree based on the 413-amino-acid Gc fragment of the CaCV-Pkk isolate supported the existence of three distinct CaCV clades. Vigna unguiculata produced concentric rings useful for discriminating the Thai CaCV peanut isolates from tomato or pepper isolates. By using reverse transcription polymerase chain reaction with species-specific primers, the three tospoviruses could be detected in mixed infections in watermelon and Physalis, as well as in the bodies of thrips vectors, Thrips palmi and Scirtothrips dorsalis, collected from fields.
对从泰国不同地区田间采集的与番茄、辣椒、葫芦科植物、花生和酸浆属植物相关的28种番茄斑萎病毒分离株进行了鉴定。基于N基因和蛋白质序列关系，鉴定出三种番茄斑萎病毒，即西瓜银斑驳病毒（WSMoV）、辣椒褪绿病毒（CaCV）和甜瓜黄斑病毒（MYSV）。对选定的N蛋白序列进行CLUSTAL分析，结果显示不同的CaCV分离株位于三个不同的进化枝中。基于番茄上的坏死症状以及它们与其他两个进化枝中CaCV分离株93%的同一性，CaCV-TD8、CaCV-AIT和CaCV-KS16（泰国番茄斑萎病毒）被指定为CaCV-番茄坏死株系。基于CaCV-Pkk分离株413个氨基酸的Gc片段构建的系统发育树支持存在三个不同的CaCV进化枝。豇豆产生的同心环有助于区分泰国CaCV花生分离株与番茄或辣椒分离株。通过使用物种特异性引物进行逆转录聚合酶链反应，可以在西瓜和酸浆的混合感染以及从田间采集的蓟马传播介体棕榈蓟马和西花蓟马体内检测到这三种番茄斑萎病毒。
