Panyasiri Cheerapha, Supothina Sumalee, Veeranondha Sukitaya, Chanthaket Rungtiwa, Boonruangprapa Tanapong, Vichai Vanicha
National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathum Thani 12120, Thailand.
Insects. 2022 Jul 28;13(8):684. doi: 10.3390/insects13080684.
In a laboratory assay, it was shown that BCC48145, BCC2660, and TBRC10638 were the three strains that exhibited the highest insecticidal activity against chili thrips, causing 92.5% and 91.86% and 92.3% corrected mortality, respectively. The fungi BCC48145 and TBRC10638 were selected for greenhouse spraying. Cytotoxicity test of the extracts from both fungi evaluated against 4 animal cell lines: KB; human oral cavity carcinoma, MCF7; human breast adenocarcinoma, NCI-H187; human small cell lung carcinoma and GFP-expressing Vero cells, showed none-cytotoxic to all cell lines. An efficacy validation in the greenhouse showed that TBRC 10638 was more effective than BCC48145 and could control the thrips up to 80% when using the fungus at 10 spores/mL. The LC values of TBRC 10638 against chili thrips based on total thrips count from two experiments were 1.42 × 10 and 1.12 × 10 spores/mL when the fungal spores were sprayed once a week. The optimal concentration of TBRC 10638 spores for effective control of chili thrips was determined at 1.41 × 10 spores/mL. The average efficacy of TBRC 10638 for thrips control from 3 field trials was 30.08%, 14.39%, and 29.92%. This result was not significantly different from that of the chemical insecticide treatment group, which showed efficacy at 19.27%, 14.92%, and 19.97%. Furthermore, there was no difference in productivity among the different treatment groups. Our results demonstrated that TBRC 10638 is a promising biocontrol agent that could be used as an alternative to chemical insecticide for controlling chili thrips.
在实验室测定中，结果表明BCC48145、BCC2660和TBRC10638这三株菌株对辣椒蓟马表现出最高的杀虫活性，校正死亡率分别为92.5%、91.86%和92.3%。选择真菌BCC48145和TBRC10638进行温室喷洒。对这两种真菌提取物针对4种动物细胞系进行细胞毒性测试：KB（人口腔癌）、MCF7（人乳腺腺癌）、NCI-H187（人小细胞肺癌）和表达绿色荧光蛋白的Vero细胞，结果显示对所有细胞系均无细胞毒性。温室中的功效验证表明，TBRC 10638比BCC48145更有效，当使用10个孢子/毫升的真菌时，可将蓟马控制率高达80%。基于两次实验中蓟马总数，TBRC 10638对辣椒蓟马的LC值，在每周喷洒一次真菌孢子时分别为1.42×10和1.12×10个孢子/毫升。确定有效控制辣椒蓟马的TBRC 10638孢子的最佳浓度为1.41×10个孢子/毫升。来自3次田间试验的TBRC 10638对蓟马控制的平均功效分别为30.08%、14.39%和29.92%。该结果与化学杀虫剂处理组的结果无显著差异，化学杀虫剂处理组的功效分别为19.27%、14.92%和19.97%。此外，不同处理组之间的生产力没有差异。我们的结果表明，TBRC 10638是一种有前景的生物防治剂，可作为化学杀虫剂的替代品用于控制辣椒蓟马。
