Adachi Ryota, Wakabayashi Tokumitsu, Oda Naoko, Shingai Ryuzo
Laboratory of Bioscience, Faculty of Engineering, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan.
Neurosci Res. 2008 Mar;60(3):300-6. doi: 10.1016/j.neures.2007.11.010. Epub 2007 Dec 8.
The chemotaxis behaviors of the nematode Caenorhabditis elegans cultivated at various temperatures (15 degrees C, 20 degrees C and 25 degrees C) were examined at various temperatures (10 degrees C, 15 degrees C, 20 degrees C and 25 degrees C) to determine the multi-sensory integration of physical (thermal) and chemical sensory information within its nervous system. Chemotaxis behavior toward sodium acetate and ammonium chloride were differently affected by both assay and cultivation temperatures, suggesting that the temperature effect on chemotaxis is not general, but rather distinctive for each chemosensory pathway. Since thermosensory cues are likely encountered constantly in C. elegans, we supposed that the chemotaxis behaviors of worms are achieved by the integration of chemo- and thermosensory information. To verify the possible contribution of thermosensory function in chemotaxis, we examined the chemotaxis behaviors of ttx-1(p767) mutant worms with defective AFD thermosensory neurons. The chemotaxis behaviors toward sodium acetate or ammonium chloride of mutant worms cultivated at 20 degrees C and 25 degrees C were reduced relative to those of wild-type worms. These results indicate the important role of multi-sensory integration of chemosensory and thermosensory information in chemotaxis behavior of the C. elegans.
在不同温度（10℃、15℃、20℃和25℃）下检测了在不同温度（15℃、20℃和25℃）培养的秀丽隐杆线虫的趋化行为，以确定其神经系统内物理（热）和化学感觉信息的多感官整合。对醋酸钠和氯化铵的趋化行为受检测温度和培养温度的影响不同，这表明温度对趋化的影响并非普遍存在，而是每种化学感觉途径都有其独特性。由于秀丽隐杆线虫可能经常遇到热感觉线索，我们推测线虫的趋化行为是通过化学和热感觉信息的整合实现的。为了验证热感觉功能在趋化中的可能作用，我们检测了具有缺陷的AFD热感觉神经元的ttx-1(p767)突变体线虫的趋化行为。与野生型线虫相比，在20℃和25℃培养的突变体线虫对醋酸钠或氯化铵的趋化行为有所降低。这些结果表明化学感觉和热感觉信息的多感官整合在秀丽隐杆线虫趋化行为中起着重要作用。
