Hammond Abdulrahman S, McConkey Samuel J, Hill Philip C, Crozier Sarah, Klein Michèl R, Adegbola Richard A, Rowland-Jones Sarah, Brookes Roger H, Whittle Hilton, Jaye Assan
Bacterial and Viral Diseases Programme, Medical Research Council Laboratories, Fajara, Banjul, The Gambia, West Africa.
J Infect Dis. 2008 Jan 15;197(2):295-9. doi: 10.1086/524685.
Antimycobacterial T cell reactivity at different stages of HIV infection was investigated. Subjects were screened with purified protein derivative (PPD), early secreted antigenic target (ESAT)-6, and culture filtrate protein (CFP)-10 antigens for interferon (IFN)-gamma-producing effector T cell responses by direct ex vivo enzyme-linked immunospot (ELISpot) assay. The proportion of responders to PPD tuberculin decreased with a reduction in CD4 T cell count, whereas the proportion of responder to ESAT-6 and CFP-10 did not. The main sources of IFN-gamma secretion were CD4 cells, and the relative responses to ESAT-6 and CFP-10 significantly increased in HIV-infected patients with decreasing CD4 cell count. This may reflect early signs of reactivation, reinfection, or a restricted, inefficient immune response to Mycobacterium tuberculosis.
研究了HIV感染不同阶段的抗分枝杆菌T细胞反应性。通过直接离体酶联免疫斑点（ELISpot）试验，用纯化蛋白衍生物（PPD）、早期分泌抗原靶标（ESAT）-6和培养滤液蛋白（CFP）-10抗原对受试者进行筛查，以检测产生干扰素（IFN）-γ的效应T细胞反应。对PPD结核菌素反应者的比例随CD4 T细胞计数的减少而降低，而对ESAT-6和CFP-10反应者的比例则没有。IFN-γ分泌的主要来源是CD4细胞，在CD4细胞计数降低的HIV感染患者中，对ESAT-6和CFP-10的相对反应显著增加。这可能反映了结核分枝杆菌再激活、再感染的早期迹象，或对结核分枝杆菌的受限、低效免疫反应。
