Han Binbing, Karim M Nazmul
Department of Chemical Engineering, Texas Tech University, Lubbock, Texas 79409, USA.
Scanning. 2008 Mar-Apr;30(2):213-20. doi: 10.1002/sca.20081.
The cytotoxicity of fullerene C60 particles on two mammalian cell lines, i.e. the Chinese hamster ovary (CHO) cells and the Madin-Darby canine kidney (MDCK) cells, has been investigated. Although innate fullerene particles have a very low solubility in deionized (DI) water, these particles can be dissolved in the tetrahydrofuran (THF) solvent at a great value. Further, the dissolved fullerene particles in the THF solvent could be extracted into a DI water solution at a significantly increased solubility. The formation of fullerene particle aggregates is believed to be the cause of the increased solubility. Results presented here show that once the concentration of the fullerene aggregates reaches a certain level, the cells start to die. The lethal dosage LD50, which is defined as the lowest fullerene concentration that results in a 50% cell death within 24 h, has been determined. Furthermore, the percentage of cell mortality increased with increasing fullerene concentration and incubation time yielding a negative effect on cell viability. These results, illustrated by atomic force microscopy (AFM), dynamic light scattering (DLS) and other microscopic techniques, will help to better understand the side effects of fullerene particles in mammalian cells.
已对富勒烯C60颗粒对两种哺乳动物细胞系，即中国仓鼠卵巢（CHO）细胞和马德堡-达比犬肾（MDCK）细胞的细胞毒性进行了研究。尽管天然富勒烯颗粒在去离子（DI）水中的溶解度非常低，但这些颗粒在四氢呋喃（THF）溶剂中具有很高的溶解度。此外，溶解在THF溶剂中的富勒烯颗粒可以以显著增加的溶解度萃取到去离子水溶液中。富勒烯颗粒聚集体的形成被认为是溶解度增加的原因。此处给出的结果表明，一旦富勒烯聚集体的浓度达到一定水平，细胞就开始死亡。已经确定了致死剂量LD50，其定义为在24小时内导致50%细胞死亡的最低富勒烯浓度。此外细胞死亡率百分比随着富勒烯浓度和孵育时间的增加而增加，对细胞活力产生负面影响。这些由原子力显微镜（AFM）、动态光散射（DLS）和其他显微镜技术所阐明的结果，将有助于更好地理解富勒烯颗粒在哺乳动物细胞中的副作用。
