Slomczynska U, Albericio F, Cardenas F, Giralt E
Departament de Quimica Organica, Facultat de Quimica, Universitat de Barcelona, Spain.
Biomed Biochim Acta. 1991;50(10-11):S67-73.
Four different supports: polyacrylamide (I), polyethylene glycol-polystyrene MP-PEG (II), Kel-F-g-styrene (III) and controlled pore glass CPG (IV-VI) were tested in order to determine the most suitable carrier for both chemical and enzymatic methods. As a model of enzymatic reaction for comparative study of supports, the hydrolysis of peptide methyl esters bound to insoluble polymer was examined. Peptide segment couplings by means of papain were attempted on the CPG-support which was found to be the most suitable for performing enzymatic reactions. The racemization-free assemblies of partially protected peptides on this support were achieved in moderate yields (31-54%).
聚丙烯酰胺（I）、聚乙二醇 - 聚苯乙烯MP - PEG（II）、聚三氟氯乙烯 - g - 苯乙烯（III）和可控孔径玻璃CPG（IV - VI），以确定化学法和酶法最适用的载体。作为用于载体比较研究的酶促反应模型，研究了与不溶性聚合物结合的肽甲酯的水解反应。尝试在发现最适合进行酶促反应的CPG载体上通过木瓜蛋白酶进行肽段偶联。在该载体上部分保护肽的无消旋组装以中等产率（31 - 54%）实现。
