Kono Ken, Song Haihan, Shingai Yasuhiro, Shioda Tatsuo, Nakayama Emi E
Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1, Yamada-oka, Suita-shi, Osaka 565-0871, Japan.
Virology. 2008 Apr 10;373(2):447-56. doi: 10.1016/j.virol.2007.12.022. Epub 2008 Feb 21.
Human immunodeficiency virus type 2 (HIV-2) strains vary widely in their ability to grow in Old World monkey (OWM) cells. We previously evaluated several HIV-2 isolates for their sensitivity to cynomolgus monkey (CM) TRIM5alpha, an anti-HIV factor in OWM cells, and found that viruses carrying proline at the 120th position of the capsid protein were sensitive to CM TRIM5alpha, whereas those with either alanine or glutamine were resistant. In the study presented here, we tested these HIV-2 isolates for their sensitivity to rhesus monkey (Rh) TRIM5alpha and found that both CM TRIM5alpha-sensitive and -resistant viruses were restricted by Rh TRIM5alpha. The variable region 1 of the SPRY domain of Rh TRIM5alpha appeared to be the determinant of this difference. Furthermore, a mutagenesis study showed that three amino acid residues TFP at the 339th to 341st positions of Rh TRIM5alpha are important for restricting HIV-2 strains resistant to CM TRIM5alpha.
2型人类免疫缺陷病毒（HIV-2）毒株在旧世界猴（OWM）细胞中的生长能力差异很大。我们之前评估了几种HIV-2分离株对食蟹猴（CM）TRIM5α的敏感性，CM TRIM5α是OWM细胞中的一种抗HIV因子，发现衣壳蛋白第120位携带脯氨酸的病毒对CM TRIM5α敏感，而携带丙氨酸或谷氨酰胺的病毒则具有抗性。在本文介绍的研究中，我们测试了这些HIV-2分离株对恒河猴（Rh）TRIM5α的敏感性，发现CM TRIM5α敏感和抗性病毒均受到Rh TRIM5α的限制。Rh TRIM5α的SPRY结构域可变区1似乎是这种差异的决定因素。此外，一项诱变研究表明，Rh TRIM5α第339至341位的三个氨基酸残基TFP对于限制对CM TRIM5α具有抗性的HIV-2毒株很重要。
