Tanaka T, Kaneda Y
Department of Parasitology, Tokai University School of Medicine, Kanagawa, Japan.
Tokai J Exp Clin Med. 1991 Dec;16(5-6):253-8.
An enzyme-linked immunosorbent assay (ELISA) for detecting antibody against Entamoeba histolytica was used in a survey to determine the prevalence of E. histolytica infection in the greater Tokyo area. Serum samples were collected from 1,869 randomly chosen, symptom-free volunteers (1,645 males and 224 females). Seventeen individuals (0.91%), 14 males and three females, had ELISA values that were interpreted as positive. The calculated histogram of the anti-E. histolytica IgG values, after logarithmic transformation of the original skewed values, revealed a normal (Gaussian) distribution. It was also found that the results of the ELISA were closely correlated with those obtained by an indirect immunofluorescent assay (IFA).
在一项调查中，采用酶联免疫吸附测定法（ELISA）检测抗溶组织内阿米巴抗体，以确定东京大都市区溶组织内阿米巴感染的流行情况。从1869名随机挑选的无症状志愿者（1645名男性和224名女性）中采集血清样本。17人（0.91%），14名男性和3名女性，ELISA值被判定为阳性。对原始偏态值进行对数转换后计算得到的抗溶组织内阿米巴IgG值直方图显示呈正态（高斯）分布。还发现ELISA结果与间接免疫荧光测定法（IFA）获得的结果密切相关。
