Poliak M S, Efimova T P
Antibiotiki. 1976 Jul;21(7):613-7.
Actively multiplicating cells of C1. perfringens proved to be more sensitive to 7-chlor-7-desoxylincomycin and rifampicin than the cells in the phase of the population dying. The bactericidal effect of the antibiotics on Clostridia vegetating at a temperature range within 37--4degrees was studied. Determination of the content of higher fatty acids in the cultivation medium with the method of gas chromatography showed that the metabolic processes in the bacterial cells went on at a temperature of 4degrees. Sensitivity of Clostridia to antibiotics at 20 and 4degrees lowered. However, all antibiotics inhibited the cell viability under such conditions. The inhibitors of the intracellular protein synthesis, i. e. rifampicin, 7-chlor-7-desoxylincomycin and morphocycline proved to be most active. The effect of beta-lactame antibiotics, i. e. cephaloridine and benzylpenicillin was reliable though lower.
事实证明，与处于群体死亡阶段的细胞相比，产气荚膜梭菌的活跃增殖细胞对7-氯-7-去氧林可霉素和利福平更敏感。研究了抗生素在37 - 4℃温度范围内对生长的梭菌的杀菌作用。用气相色谱法测定培养基中高级脂肪酸的含量表明，细菌细胞中的代谢过程在4℃温度下仍在进行。梭菌在20℃和4℃时对抗生素的敏感性降低。然而，在这种条件下所有抗生素均抑制细胞活力。细胞内蛋白质合成抑制剂，即利福平、7-氯-7-去氧林可霉素和吗啉环素被证明最具活性。β-内酰胺类抗生素，即头孢菌素和苄青霉素的作用虽然较弱但很可靠。
