Pires Ana Rita, Araújo Alberto N, Montenegro M Conceição B S M, Chocholous Petr, Solich Petr
Requimte, Dep. de Química Física, Faculdade de Farmácia, (UP) Rua Aníbal Cunha 164, 4099-030 Porto, Portugal.
J Pharm Biomed Anal. 2008 Mar 13;46(4):683-91. doi: 10.1016/j.jpba.2007.12.003. Epub 2007 Dec 8.
The construction, evaluation and analytical application of potentiometric sensors sensitive to vitamin B1 and vitamin B6 are reported. The solid contact electrodes were produced using beta-cyclodextrins as ionophores in a carboxylated poly(vinyl chloride) support matrix. Near Nernstian slopes (mV/decade) of 51.7+/-0.8, 60.6+/-0.6 and 61.1+/-1.4, within the intervals (M) of 1.0 x 10(-4) to 1.0 x 10(-1), 5.8 x 10(-5) to 1.0 x 10(-1) and 4.3 x 10(-5) to 1.0 x 10(-1) were obtained, for thiamine and pyridoxine I and II prepared membranes, respectively. A pH operational range of 6.5-8.5 for thiamine and 2-4.5 for pyridoxine electrodes was found. Assessment of selectivity coefficients toward a large number of inorganic cations and organic cations usually present in multivitamin formulations revealed good performance. Analysis of vitamins B1 and B6 in complex multivitamin drugs was achieved with recoveries within the intervals of 95.1-99.6% for thiamine and 95.1-102% for pyridoxine. Furthermore, the results enabled by the proposed procedure revealed good agreement with those provided by HPLC.
报道了对维生素B1和维生素B6敏感的电位传感器的构建、评估及分析应用。采用β-环糊精作为离子载体，在羧化聚氯乙烯支撑基质中制备了固体接触电极。对于硫胺素和吡哆醇I及II制备的膜，在1.0×10(-4)至1.0×10(-1)（M）、5.8×10(-5)至1.0×10(-1)和4.3×10(-5)至1.0×10(-1)的区间内，分别获得了接近能斯特斜率（mV/十年）为51.7±0.8、60.6±0.6和61.1±1.4。发现硫胺素电极的pH操作范围为6.5 - 8.5，吡哆醇电极的pH操作范围为2 - 4.5。对多种维生素制剂中通常存在的大量无机阳离子和有机阳离子的选择性系数评估显示出良好的性能。在复合多种维生素药物中对维生素B1和B6进行分析，硫胺素的回收率在95.1 - 99.6%之间，吡哆醇的回收率在95.1 - 102%之间。此外，所提出的方法得到的结果与高效液相色谱法提供的结果显示出良好的一致性。
