Ferrini Anna Maria, Mannoni Veruscka, Carpico Graziella, Pellegrini Guido Enrico
National Centre for Food Quality and Risk Assessment, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
J Agric Food Chem. 2008 Feb 13;56(3):784-8. doi: 10.1021/jf071479i. Epub 2008 Jan 23.
A novel application of a hybrid biosensor is here employed as an analytical method for the detection and presumptive identification of beta-lactam residues in milk. The method is based on measurements of carbon dioxide (CO2), the production of which is related to the microbial growth of the test microorganism Bacillus stearothermophilus var. calidolactis. The presence of beta-lactams in milk inhibits microbial growth and, consequently, the CO2 production rate. The analysis is based on the variation of CO2 between a milk sample spiked with beta-lactams and a twin milk sample containing beta-lactams plus a broad spectrum beta-lactamase, using an electrochemical device of biosensor. A blank milk sample is included as control. The result is obtained starting from the first 120 min. Moreover, the ability to recognize all of the beta-lactams speeds the total time of analysis when chemical identification and quantification are required. The analytical method appears to be adequate for milk control for qualitative screening purposes, complying with the requirements stated in Decision 2002/657/EC.
本文采用一种新型混合生物传感器作为分析方法，用于检测和初步鉴定牛奶中的β-内酰胺残留。该方法基于对二氧化碳（CO₂）的测量，CO₂的产生与测试微生物嗜热脂肪芽孢杆菌嗜热栖热亚种的微生物生长有关。牛奶中β-内酰胺的存在会抑制微生物生长，从而抑制CO₂的产生速率。该分析基于使用生物传感器的电化学装置，对比添加了β-内酰胺的牛奶样品与含有β-内酰胺加广谱β-内酰胺酶的双份牛奶样品之间CO₂的变化。包含一份空白牛奶样品作为对照。结果从最初的120分钟开始获取。此外，当需要进行化学鉴定和定量时，识别所有β-内酰胺的能力加快了总分析时间。该分析方法似乎足以用于牛奶的定性筛查控制，符合2002/657/EC号决定中规定的要求。
