抗坏血酸、金属离子与超氧自由基。
Ascorbic acid, metal ions and the superoxide radical.
作者信息
Halliwell B, Foyer C H
出版信息
Biochem J. 1976 Jun 1;155(3):697-700. doi: 10.1042/bj1550697.
Abstract
- No evidence could be found for production of the superoxide radical, O2-, during autoxidation of ascorbic acid at alkaline pH values. Indeed, ascorbate may be important in protection against O2- genat-d in vivo. 2. Oxidation of ascorbate at pH 10.2 was stimulated by metal ions. Stimulation by Fe2+ was abolished by superoxide dismutase, probably because of generation of O2-- during reduction of O2 by Fe2+, followed by reaction of O2-- with ascorbate. EDTA changed the mechanism of Fe2+-stimulated ascorbate oxidation. 3. Stimulation of ascorbate oxidation by Cu2+ was also decreased by superoxide dismutase, but this appears to be an artifact, since apoenzyme or bovine serum albumin showed similar effects.
摘要
- 在碱性pH值下抗坏血酸自动氧化过程中，未发现产生超氧阴离子自由基O₂⁻的证据。实际上，抗坏血酸盐在体内抵御O₂⁻生成方面可能很重要。2. 在pH 10.2时，金属离子会刺激抗坏血酸盐的氧化。超氧化物歧化酶可消除Fe²⁺的刺激作用，这可能是因为Fe²⁺将O₂还原过程中生成了O₂⁻，随后O₂⁻与抗坏血酸盐发生了反应。EDTA改变了Fe²⁺刺激抗坏血酸氧化的机制。3. 超氧化物歧化酶也会降低Cu²⁺对抗坏血酸氧化的刺激作用，但这似乎是一种假象，因为脱辅酶或牛血清白蛋白也有类似作用。
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