Little H N, Jones O T
Biochem J. 1976 May 15;156(2):309-14. doi: 10.1042/bj1560309.
The etioplast fraction prepared from dark-grown barley contained the enzyme ferrochelatase. A mitochondrial fraction prepared from the same dark-grown tissue also contained ferrochelatase. After density-gradient centrifugation an etioplast band was collected that was free from detectable mitochondrial marker enzymes and yet retained ferrochelatase activity. A membrane band that was enriched in mitochondria also contained ferrochelatase. The ferrochelatase in these two bands had different pH optima, but appeared very similar in their porphyrin specificity and their inhibition by metalloporphyrins.
从黑暗生长的大麦中制备的黄化质体部分含有铁螯合酶。从相同黑暗生长组织中制备的线粒体部分也含有铁螯合酶。经过密度梯度离心后，收集到一条不含可检测到的线粒体标记酶但仍保留铁螯合酶活性的黄化质体带。一条富含线粒体的膜带也含有铁螯合酶。这两条带中的铁螯合酶具有不同的最适pH值，但在卟啉特异性和金属卟啉对其的抑制作用方面似乎非常相似。
