Sun Yuhua, Tang Rong, Li Dapeng, Zhang Xuzhen, Fu Juan, Xie Ping
Key Laboratory of Animal Development, Genetics and Reproduction of Education Ministry, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Environ Toxicol. 2008 Apr;23(2):145-52. doi: 10.1002/tox.20317.
Recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mug MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxidant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxidant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxidant enzyme genes.
最近的证据表明，氧化应激可能在微囊藻毒素毒性的发病机制中起重要作用。在本研究中，以每千克体重腹腔注射50微克MC-LRq的方式，研究了微囊藻毒素对鲫鱼肝脏中抗氧化酶基因转录的急性影响。我们报道了四种抗氧化酶（谷胱甘肽过氧化物酶、过氧化氢酶、铜/锌超氧化物歧化酶和谷胱甘肽还原酶）基因的cDNA序列，并通过实时PCR方法分析抗氧化酶基因的转录丰度，评估微囊藻毒素诱导的氧化应激。在1、3、12、24和48小时测定抗氧化酶基因相对转录丰度和表达的时间依赖性变化。抗氧化酶之间的转录丰度各不相同，谷胱甘肽过氧化物酶和谷胱甘肽还原酶下调，而过氧化氢酶和超氧化物歧化酶显著上调。基于这些数据，我们初步得出结论，微囊藻毒素诱导了氧化应激，并导致抗氧化酶基因的不同反应。
