Bi Xiao-bin, Deng Yu-bin, Gan Dan-hui, Wang Ya-zhu
Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
Acta Pharmacol Sin. 2008 Feb;29(2):169-76. doi: 10.1111/j.1745-7254.2008.00710.x.
Stem cells hold great promise for brain and spinal cord injuries (SCI), but cell survival following transplantation to adult central nervous system has been poor. Salvianolic acid B (Sal B) has been shown to improve functional recovery in brain-injured rats. The present study was designed to determine whether Sal B could improve transplanted mesenchymal stem cell (MSC) survival in SCI rats.
SCI rats were treated with Sal B. The Basso-Beatie-Bresnahan (BBB) scale was used to test the functional recovery. Sal B was used to protect MSC from being damaged by TNF-alpha in vitro. Bromodeoxyuridine-labeled MSC were transplanted into SCI rats with Sal B intraperitoneal injection, simultaneously. MSC were examined, and the functional recovery of the SCI rats was tested.
Sal B treatment significantly reduced the lesion area from 0.26+/-0.05 mm2 to 0.15+/-0.03 mm2 (P<0.01) and remarkably raised the BBB scores on d 28, post-injury, from 7.3+/-0.9 to 10.5+/-1.3 (P<0.05), compared with the phosphate-buffered saline (PBS) control group. MSC were protected from the damage of TNF-alpha by Sal B. The number of surviving MSC in the MSC plus Sal B groups were 1143.3+/-195.6 and 764.0+/-81.3 on d 7 and 28, post-transplantation, more than those in the MSC group, which was 569.3+/-72.3 and 237.0+/-61.3, respectively (P<0.05). Rats with MSC transplanted and Sal B injected obtained higher BBB scores than those with MSC transplanted alone (P<0.05) and PBS (P<0.01).
Sal B provides neuroprotection to SCI and promotes the survival of MSC in vitro and after cell transplantation to the injured spinal cord in vivo.
干细胞对脑和脊髓损伤具有巨大的应用前景，但移植到成年中枢神经系统后的细胞存活率一直很低。丹酚酸B（Sal B）已被证明可改善脑损伤大鼠的功能恢复。本研究旨在确定Sal B是否能提高脊髓损伤大鼠移植间充质干细胞（MSC）的存活率。
对脊髓损伤大鼠给予Sal B治疗。采用Basso-Beattie-Bresnahan（BBB）评分量表测试功能恢复情况。在体外，用Sal B保护MSC免受肿瘤坏死因子-α（TNF-α）的损伤。将溴脱氧尿苷标记的MSC腹腔注射到接受Sal B治疗的脊髓损伤大鼠体内，同时进行移植。对MSC进行检测，并测试脊髓损伤大鼠的功能恢复情况。
与磷酸盐缓冲盐水（PBS）对照组相比，Sal B治疗显著将损伤面积从0.26±0.05平方毫米减少至0.15±0.03平方毫米（P<0.01），并在损伤后第28天显著提高BBB评分，从7.3±0.9提高到10.5±1.3（P<0.05）。Sal B保护MSC免受TNF-α的损伤。移植后第7天和第28天，MSC加Sal B组存活的MSC数量分别为1143.3±195.6和764.0±81.3，多于MSC组的569.3±72.3和237.0±61.3（P<0.05）。注射Sal B并移植MSC的大鼠获得的BBB评分高于单独移植MSC的大鼠（P<0.05）和PBS组（P<0.01）。
Sal B对脊髓损伤具有神经保护作用，并在体外及细胞移植到体内损伤脊髓后促进MSC的存活。
